摘要
目的探讨单核源巨噬细胞分泌的基质金属蛋白酶-9(MMP-9)、组织因子(TF)在冠心病中的临床意义。方法密度梯度离心法提取12名急性心肌梗死(AM I)、16名不稳定心绞痛(UAP)、12名稳定型心绞痛(SAP)患者和8名冠脉正常者(Norm)的单核细胞,用佛波酯诱导其分化为巨噬细胞,ELISA法测定单核源巨噬细胞上清液MMP-9、TF含量。结果AM I、UAP组单核源巨噬细胞体外分泌MMP-9、TF的含量明显高于SAP、Norm组(P<0.05和<0.01),但AM I与UAP组间、SAP与Norm组间均无统计学差异,且MMP-9、TF含量与年龄、冠脉病变积分无统计学相关,在不同冠心病危险因素组间(0、1-2、3-4、4个以上危险因素)亦无统计学差异。结论单核源巨噬细胞体外分泌MMP-9、TF可作为急性冠脉综合征(ACS)病情评估的参考指标。
Objective To investigate the clinical significance of matrix metalloproteinase - 9 （MMP - 9） and tissue factor （TF） secreted by monocyte derived- macrephages in vitro in coronary heart disease （CHD）. Methods Monocytes in peripheral blood were obtained from 12 patients with acute myocardial infarction（ AMI）, 16 unstable angina pectoris （UAP）,12 stable angina pectoris （SAP） and 8 normals （Norm） by ficoll density gradient centrifugalization. The cells were incubated for 48 hours to induce them to differentiate into macrephages by adding phorbolester 12 - myristate 13 - acetate （PMA）. The levels of MMP - 9 and TF in the supematant were measured by ELISA. Results It was found that the levels of MMP -9 and TF were significantly higher in AMI and UAP groups than those in SAP and Norm groups （P 〈0.05 and P 〈0.01 respectively）. But there were no significant differences in them between AMI group and UAP group,or between SAP group and Norm group. Moreover, nor were there any statistical differences in the levels of MMP -9 and TF among the different CHD risk factor ranks（0, 1 -2, 3 -4 and more than 4 risk factors）, or any correlation of the MMP - 9 and TF levels to the age and the coronary artery score. Conclusion The levels of MMP - 9 and TF secreted by macrephages in patients with CHD in vitro could be referred to as an index to estimate the condition of acute coronary syndrome （ACS）.
出处
《徐州医学院学报》
CAS
2007年第2期74-77,共4页
Acta Academiae Medicinae Xuzhou
基金
江苏省中医药管理局资助课题(H05105)
关键词
冠心病
单核源巨噬细胞
基质金属蛋白酶-9
组织因子
coronary heart disease
monocyte -derived macrophages
matrix metalloproteinase -9
tissue factor
