摘要
目的:分离菜籽清蛋白水解物并研究混合肽及级分对大鼠血管紧张素Ⅰ转换酶(ACE)活性的影响。方法:采用葡聚糖凝胶过滤色谱(SephadexG-25)分离菜籽肽;离体培养检测血管紧张素Ⅰ转换酶(ACE)活性。结果:菜籽肽(RSP-R)及分离的三个级分(RSP-Ⅰ、Ⅱ和Ⅲ)对高血压大鼠ACE活性均有明显抑制作用,其中级分Ⅲ抑制能力最强。结论:菜籽清蛋白水解多肽具有抑制ACE活性能力,这一结果有助于菜籽清蛋白的广泛应用。
Objective ：To isolate the Rapeseed Albumin hydrolysatc and study the effects on the activity of angiotcnsion I - converting enzyme（ACE） of the rapeseed peptides on rats and the grades. Methods： ①Using Sephadex G-25 column with water as eluant; ② Spontaneously hypertensive rats（SHR） were used as animal model, the changes of ACE activity were investigated in vitro. Results ：Three purified grades were isolatod（RSP- Ⅰ ,Ⅱ, and Ⅲ） by Sephadex G-25 column. RSP-R and three fractions exert remarkable inhibition on ACE activity, among which RSP-3 showed the highest inhibition. Conclusion： Proteolysis of rapeseed albumin can result ACE inhibitory activity in peptides. The high quality of RSP can be used directly in the supplementation of foods and can also widen the field of application of rapeseed albumin.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2007年第2期289-292,共4页
Food Science
基金
天津大学青年教师基金项目(5110107)
国家"十五"科技重点攻关计划项目(2001BA501A05)
