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荧光PCR法检测鸡肉中沙门氏菌的研究 被引量:3

Detection of Salmonella in Chicken with Fluorescence Polymerase Chain Reaction
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摘要 作者采用合成的基因探针和引物,对2株阳性标准菌株2、株野生菌株和1个无菌去离子水进行检测,结果准确可靠,整个试验过程在30 h内完成。可见,该法是一种特异、敏感、简便、快速的沙门氏菌检测方法,对快速通关将发挥积极的作用。 In present article, there are two positive standard salmonella bacteria. two positive sample about chicken and a sample of aseptic water. This samples are detected with fluorescence polymerase chain reaction. The results showed: The method is efficient and accurate. Furthermore. it took only 30 hours for whole PCR process. It is concluded that PCR is a specific, sensitive, rapid. simple and convenient method for detection of salmonella. It may have great values for the entry-exit inspection and quarantine.
出处 《中国畜牧兽医》 CAS 2007年第2期96-97,共2页 China Animal Husbandry & Veterinary Medicine
关键词 沙门氏菌 鸡肉 荧光PCR salmonella chicken fluorescence PCR
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参考文献5

  • 1Anh V N.Avian Diseases,1994,38:119.
  • 2Bassler H A,Flood S J A,Livak K J.Use of a Fluorogenic probe in a PCR-Based Assay for the Detection of Listeria monocytogenes.Applied And Environmental MicroBiology,1995,61:3724~3728.
  • 3Chen S,Yee A,Griffiths M.The evaluation of a fluorogenic polymerase chain reaction assay for the detection of Salmonella species in food commodities.Int J Food Microbiol,1997,35:239~250.
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  • 5Rahn K,De G S A,Clarke R C.Amplification of an invA gene sequence of salmonella typhimurium by polymerase chain reaction as a specific method of detection of salmonella.Mol Cell Probes,1992,6:271~279.

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