摘要
目的:建立检测人血浆甲砜霉素的高效液相色谱法,并对其在人体内的药代动力学进行研究。方法:以 ZORBAX SB-C_(18)(4.6 mm×250 mm,5μm)为色谱柱;乙腈-水(30:70)为流动相,流速0.8 mL·min^(-1);以10,11-二氢-10-羟基-卡马西平(MHD)为内标,血浆样品经乙酸乙酯和正己烷提取后,在225 nm 波长下进行检测。结果:甲砜霉素在0.1～10.0 mg·L^(-1)浓度范围内线性关系良好(r=0.9999),定量下限为0.1 mg·L^(-1);0.25,1.0,5.0μg·mL^(-1)3种浓度的日间、日内精密度RSD(n=5)均小于10%;相对回收率均在85%～115%范围内,绝对回收率大于75%。结论:本方法操作简便,结果准确;适用于血浆甲砜霉素的检测。
Objective：To establish an HPLC method for the determination of thiamphenicol in human plasma and study of its pharmacokinetics in Chinese volunteers. Method：The analytical column was packed with ZORBAX SB -C18 （4. 6 mm × 250 mm ,5 μm） ,the mobile phase was acetonitrile -water （30： 70） ,the flow rate was 0. 8 mL ·min^-1, and detection wavelength was set at 225 nm, respectively. Plasma samples were extracted with ethyl acetate and n - hexane. Results： The linear range of thiamphenicol in human plasma was 0. 1 - 10. 0 mg · L^-1 （ r = 0. 9999）. The lowest limit was 0. 1 mg · L^-1 ; Intra - day and inter - day RSD for the analysis of thiamphenicol in plasma samples at three concentration levels were both lower than 10%. Relative and absolute recoveries were 85% -115% and more than 75%, respectively. Conclusion： The HPLC method is simple, high selective and sensitive for the determination of thiamphenicol in plasma.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2007年第2期177-179,共3页
Chinese Journal of Pharmaceutical Analysis
