摘要
研究了一种L-抗坏血酸脂肪酸酯（AFAE）合成新途径。实验结果表明，氢化棕榈油、大豆油和海狗油等食用油脂作为脂肪酸基团供体，在固定化脂肪酶的催化下，在有机溶荆中与L-抗坏血酸直接进行酯交换反应形成L-抗坏血酸脂肪酸酯。对反应介质体系的筛选发现，在叔戊醇中用Novozym435催化此反应所得的产物浓度最高。对影响合成抗坏血酸脂肪酸酯的因素进行了探讨，确定了最适反应条件：初始油脂底物浓度为200-600mmol／L，温度为55℃，反应时间为9h。此条件下产物质量浓度可达43．51g／L。
A novel route for synthesis of L-ascorbyl fatty acid esters （AFAE） was investigated. The experiment results show that edible oilsuch as hardened palm oil, soybean oil and Namibian seal＇s oilcan be used as the donor of fatty acid acyl group to synthesize the AFAE via transesterification with L-ascorbic acid in organic media catalyzed by immobilized lipase. Through screening of reaction media and lipase, it was found that the highest product concentration could be achieved by Novozym435 in tert-amyl alcohol. The influence of substrate concentration, reaction time etc. on the synthesis of L-ascorbic acid esters were also investigated and the reaction conditions were optimized. For hardened palm oil, the optimum reaction conditions were as follow： reaction time 9 hours under 55 ℃ with the initial concentration of oil 200-600 mmol/ L. By combinational of optimal reaction conditions, the product concentration could be achieved at 43.51 g/L.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2007年第1期100-105,共6页
Journal of Food Science and Biotechnology
关键词
抗坏血酸脂肪酸酯
酶法合成
脂肪酶
有机溶剂
ascorbyl fatty acid esters （AFAE）
enzymaticsynthesis
lipase
organic solvents
