摘要
从番茄果实表面分离到一株陆生伊萨酵母（Issatchenkia terricola），它在几丁质诱导下能产生较高活性的几丁质酶。采用硫酸铵盐析、DEAE-纤维素（DE32）离子交换柱层析、SephadexG-100分子筛柱层析，获得了凝胶电泳均一的几丁质酶。用SDS-PAGE测得该酶的相对分子质量为42400；以胶体几丁质为底物的Km和Vmax分别为6．91mg／mL和12．95μmol／min；该酶反应的最适温度和最适pH值分别为50℃和7．0。在50℃条件下，酶蛋白在pH5．0～9．0较为稳定；抗菌活性实验表明，该酶对供试病原菌有不同程度的抑制作用。
Issatchenkia terricola, a yeast could produce quite high active chitinase when induced by chitin, was isolated from the surface of tomato fruits. The enzyme was purified to electrophoretic homogeneity by the steps of ammonium sulfate precipitation, DEAE-cellulose ion exchange and SephadexG-100 gel filtration chromatography. The molecular weight of chitinase was estimated to be 42 400 by SDS-PAGE. The Km and Vmax values of the enzyme on colloidal chitin were 6.91 mg/mL and 12.95 μmol/min, respectively. The optimum pH was 7.0 and the optimum temperature was 50 ℃. The enzymatic activity was stable under 50 ℃ and at the pH range of 5.0 -9.0. The enzyme showed antifungal activity against tested fungus to different degree.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2007年第1期90-94,共5页
Journal of Food Science and Biotechnology
基金
河南省自然科学基金项目(0411032300)
关键词
陆生伊萨酵母
几丁质酶
纯化
性质
抗菌活性
Issatchenkia terricola
chitinase
purification
properties
antifungal activity
