摘要
采用响应面方法对一株能够选择性水解(R)-烯丙醇酮乙酸酯的菌株的发酵产酶培养基进行了优化。首先考察了不同碳、氮源的影响,在此基础上通过Plackett-Burman设计法对发酵培养基主要因子进行筛选,确定主要影响因子为橄榄油的质量浓度,黄豆饼的质量浓度和K2HPO4的质量浓度。用最陡爬坡实验逼近以上三因素最优水平,并用中心组合设计以及响应面分析确定主要影响因子的最佳浓度。在优化培养条件下,发酵液中酶产量从94U/L提高到了358U/L,增加了281%。
Response surface methodology（RSM） was employed to optimize culture medium for the production of a hydrolase which is useful for the enantioselective hydrolysis of racemic 4-hydroxy- 3-methyl-2-（2-propenyl）-cyclopent-2-enone （rac-HMPC） acetate. The influences of carbon and nitrogen resource was investigated. Then a Plackett-Burman design was used to evaluate the effects of different components in the culture medium. Olive oil, soy bean meal and K2HPO4 have significant influences on the hydrolase production. The path of steepest ascent was used to approach the optimal region of above three factors. The concentrations of three factors were optimized subsequently using central composite designs and response surface analysis. As the result, the production of hydrolase in shake flask increased from 94U/l to 358U/l with the optimal medium. The yield is increased by 281%.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2007年第1期71-76,共6页
Journal of Food Science and Biotechnology
基金
国家自然科学基金项目(20336010)
国家"973"计划项目(2003CB716008)
