摘要
目的探讨肾母细胞瘤基因(Wilm's tumor gene,WT1)与血管内皮生长因子C(vascular endothelial growth factor C,VEGF-C)蛋白在上皮性卵巢癌中的表达、临床意义及二者的相关性。方法采用免疫组织化学SP法检测50例卵巢上皮性癌、10例卵巢良性肿瘤和10例正常卵巢石蜡标本中WT1、VEGF-C的表达情况。结果上皮性卵巢癌与卵巢良性肿瘤、正常卵巢组织相比,WT1和VEGF-C阳性表达率都增加,差异有统计学意义(χ2=9.818,P<0.01;χ2=7.061,P<0.01)。WT1阳性表达与上皮性卵巢癌的病理分级及FIGO分期相关(P<0.05),与病理类型呈极显著相关(P<0.001),而与年龄及淋巴结转移无关(P>0.05)。VEGF-C阳性表达与上皮性卵巢癌FIGO分期相关(P<0.05),与淋巴结转移呈极显著相关(P<0.001),与年龄、病理类型、病理分级无关(P>0.05)。在上皮性卵巢癌中,WT1和VEGF-C阳性表达呈正相关(γ=0.342,P<0.05)。结论WT1在不同亚型上皮性卵巢癌中表达不同。WT1异常高表达可能是诊断卵巢癌的分子标志物。WT1和VEGF-C在卵巢癌的浸润转移中起促进作用,两者共同参与上皮性卵巢癌的发生、发展。
Objective To investigate the expressions of Wilms＇ tumor gene （ WT1 ） and vascular endothelial growth factor C （VEGF-C） proteins in human epithelial ovarian carcinoma and evaluate their correlation and clinical significance. Methods： The expressions of WT1 and VEGF-C proteins in 50 cases of epithelial ovarian cancer （ EOC）, 10 cases of benign epithelial tumor （ BET）, 10 cases of normal ovarian （NO） were examined by immunohistochemical streptavidin/peroxidase method. Results：The positive expression of WT1 and VEGF-C protein in the tissues of EOC were significantly higher than those in the BET and the NO （X^2 =9. 818 ,P 〈0.01 ; X^2 = 7. 061, P 〈 0.01 ）. The positive expression of WT1 had a correlation with the histological grade and FIGO stage （P 〈 0.05 ）, and had a strong correlation with the histological type （P 〈0.001 ）. But it was not associated with the age and the lymph node metastasis （ P 〉 0.05 ）. The positive expression of VEGF-C was associated with the FIGO stage （ P 〈 0. 05 ）, and was strongly associated with lymph node metastasis （P 〈0.001 ）. But it did not correlate with the age, histological type, and histological grade （P 〉0.05）. In addition, the expression of WT1 had positive correlation with VEGF-C expression in EOC （ γ = 0. 342, P 〈 0.05 ）. Conclusion： （ 1 ） WT1 has different expressions in different histologic subtypes of primary ovarian carcinomas. （ 2 ） The overexpression of WT1 might be the molecular marker for the epithelial ovarian Carcinoma. （3） WT1 and VEGF-C increase the invasion and metastasis of the epithelial ovarian carcinoma. Both of them play important roles in the carcinogenesis and progression of epithelial ovarian carcinoma.
出处
《肿瘤》
CAS
CSCD
北大核心
2007年第2期134-138,共5页
Tumor
