摘要
目的：探讨中国北方人群中XRCC2基因C41657T、G4234C多态性与肺癌的关系。方法：应用PCR-RFLP方法检测199例肺癌患者和200例正常人的XRCC2 C41657T及G4234C多态位点，比较两组之间等位基因及基因型频率分布及其与肺癌的关系。结果：肺癌患者XRCC2 C41657T多态位点的CC、CT、TT基因型和C、T等位基因频率分布与健康对照组相比差异均无统计学意义（P〉0．05）。G4234C多态位点的GG、GC、CC基因型和G、C等位基因频率分布与健康对照组相比差异也均无统计学意义（P〉0．05）。两多态性位点联合分析显示，肺癌患者与健康对照组的4个单体型分布亦无统计学差异（P〉0．05）。以病理类型、吸烟状况和年龄进行分层分析显示，XRCC2 C41657T多态位点可能与腺鳞癌和不吸烟人群的肺癌发病风险相关；与C／C基因型相比，携带T等位基因的基因型（C／T＋T／T）可显著增加腺鳞癌的发病风险（OR为2．95，95％CI=1．15—7．59）；而C／T基因型可显著增加不吸烟组人群中肺癌的发病风险（OR为2．12，95％CI=1．05-4．27）。对于XRCC2 G4234C多态位点，与G／G基因型相比，G／C基因型和携带C等位基因的基因型（G／C＋C／C）可显著增加小细胞肺癌的发病风险（OR为2．82和2．82；95％CI=1．15～6．91和1．17～6．76）；G／C基因型或与C／C基因型相加可显著增加年龄≥60岁的人群中肺癌的发病风险（OR为2．29和2．37；95％CI=1．11—4．72和1．16—4．88）。结论：对于XRCC2 C41657T多态位点，携带T等位基因的基因型可能增加腺鳞癌的发病风险，C／T基因型可能增加不吸烟者患肺癌风险；XRCC2 G4234C多态位点，G／C基因型或携带C等位基因可能增加小细胞肺癌的发病风险和老年人（年龄≥60岁）患肺癌的风险。
Objective： To investigate the possible association of functional CA1657T and G4234C polymorphisms of XRCC2 genes with susceptibility of lung cancer in Northern Chinese population. Methods： The single nucleotide polymorphisms （SNP） in XRCC2 CA1657T and G4234C were genotyped by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） analysis in 199 patients with lung cancer, and 200 healthy controls. The genotype and allele distribution were compared and their contribution to the risk of lung cancer were analyzed. Results： The genotype （ CC, CT, and TT） and allele distribution of the XRCC2 CA1657T and G4234C SNP in the patients with lung cancer was not significantly different compared with that in healthy controls （all P 〉 0.05 ）. When the two XRCC2 SNP were combined, the haplotype distribution in lung cancer patients was not significantly different from that in healthy controls （ P 〉 0.05 ）. When stratified for histological type, smoking status, and age, compared with C/C genotype, combination of C/T and T/T genotypes of XRCC2 CA1657T increased the risk of developing adenosquamous carcinoma （ OR = 2.95, 95% CI = 1.15-7.59） , and the individuals with C/T genotype of XRCC2 C41657T had the increased risk of developing lung cancer in non smokers （ OR = 2.12,95% CI = 1.05-4.27 ）. Compared with G/G genotype, the combination of G/C and C/C of XRCC2 G4234C and G/C genotype both increased the risk of developing small cell lung cancer （ OR =2.82 and 2.82;95% CI = 1.15-6.91 and 1.17- 6.76）. For old subjects （ age≥60 years）, the combination of G/C and C/C of G4234C and G/C genotype both increased the risk of developing lung cancer （ OR = 2.29 and 2.37 ;95 % CI = 1.11-4.72 and 1.16-4.88 ）. Conclusions： For XRCC2 C41657T, both heterozygote and homozygote T carriers have the increased risk of developing adenosquamous carcinoma, the heterozygote CT carriers have the increased risk of developing lung cancer in non smokers. For G4234C, both heterozygote and homozygote C carriers have the increased risk of developing lung cancer in old subjects （ age≥60 years） and of small cell lung cancer in Northern Chinese population.
出处
《肿瘤》
CAS
CSCD
北大核心
2007年第2期123-128,共6页
Tumor
基金
河北省普通高等学校强势特色学科肿瘤学建设经费资助
