摘要
病毒的核酸检测技术发展非常迅速,主要有PCR技术、分子杂交技术、dsRNA技术、NASBA技术和基因芯片技术。综述了这些技术的原理、特点及其在果树病毒检测中的应用情况。基于PCR技术的检测方法是目前果树病毒检测的主要方法,许多果树病毒的PCR检测体系已经建立。NASBA技术也已开始用于果树病毒的检测。
Detection techniques based on nucleic acid for plant viruses,including of PCR,molecular hybridization,dsRNA,NASBA and DNA chip,were developed rapidly.The principle,characteristics and applications of these techniques in detection of fruit tree viruses are reviewed.At present,the detection methods based on PCR are the main methods for fruit tree viruses,and many fruit tree viruses have been detected by PCR.NASBA is applied to the detection of fruit tree virus recently.
作者
杨洪一
李丽丽
张志宏
Yang Hongyi;Li Lili;Zhang Zhihong(College of Life Sciences,Northeast Forestry University,Harbin 150040;College of Horticulture,Shenyang Agricultural University,Shenyang 110161)
出处
《中国农学通报》
CSCD
2007年第2期350-354,共5页
Chinese Agricultural Science Bulletin
基金
国家自然科学基金"草莓病毒分子检测研究"(30200187)
