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不同激素对春石斛的组织培养影响研究初报 被引量:9

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摘要 用春石斛(Dend robium nobile)1~3mm的茎尖为外植体,以1/2MS为基本培养基,调节不同激素水平诱导芽体萌发,进行繁殖培养。研究结果表明:萌芽诱导阶段:1/2MS+6-BA0.5mg/L+NAA0.5mg/L+椰子汁200rnL/L+蔗糖30g/L+活性碳3g/L+琼脂5g/L中萌芽早,生长快;继代和增殖培养阶段:以1/2MS+6-BA0.5mg/L+NAA2.0mg/L+椰子汁200mL/L+蔗糖15g/L+活性碳3g/L+琼脂5g/L效果最好;生根阶段培养基:1/2MS4-NAA0.1mg/L+蔗糖15g/L+活性碳3g/L+琼脂5g/L每茎段可生根4条肉质健壮,在培养过程中达到了较好的效果。
出处 《北方园艺》 CAS 北大核心 2007年第3期177-178,共2页 Northern Horticulture
基金 四川省科技攻关计划项目 编号:2006Z10-006 成都市科瑞园艺有限公司科研基金共同资助
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  • 1NIHAR R N, SHIBA P R, SATYANARAYAN P. In vitro propagation of three epiphytic orchids, Cymbidium aloi folium (L) Sw, Dendrobium aphyllum (Roxb) Fisch and Dendrobium moschatum ( Buch-Ham ) Sw.through thidiazuron-induced high frequency shoot proliferation [J]. Selentia Itor ticulturae, 1997,71 (3) : 24 -250.
  • 2LESHEM B, SHALEY D P, IZHAR S. Cytokinin as an inducer of vitrication in melon[J]. Ann Bot, 1988,61(2):255-260.
  • 3LESHEM B. Carnation plantlets from vitrified plants as a source of somaclonal variation[J]. Hortseienee,1986,21(2):320-321.
  • 4JAIN A, HUSSAIN S, KOTHARI S L. Micropropagation of Dianthus caryophyllus L control of vitrification[J]. J Plant Biochem Biotechnol, 1997,6(1) : 35-37.
  • 5WILLMER C M, PADMASREE K, RAGHAVENDRA A S. A novel method of measuring volume changes of mesophyll cell protoplasts and the effect ofmercuric chloride on their osmotically-induced swelling[J]. J Exp Bot, 1999,50(332) :401-408.
  • 6WAN Xian-chong, ZWIAZAK J J. Mercuric chloride effects on root water transport in aspen seedlings[J].Plant Physiol, 1999,121 (3) : 939-946.
  • 7TIBURCIO A F, KAUR-SAWHNEY R, INGERSOLL R B, et al. Correlation between polyamines and pyrrolidine alkaloids in developing tobacco callus [J]. Plant Physiol, 1985,78 (2) : 323 - 326.
  • 8KAUR-SAWHNEY R, SHEKHAWAT N S, GALSTON A W. Polyamine levels as related to growth,differentiation and senescence in protoplast-derived cultures of Vigna aconitifolia Avena sativa[J]. Plant Growth Regul, 1985,3 : 329-337.
  • 9TIBURCIO A F, KAUR-SAWHNEY R, GAL-STON A W. Effect of polyamine biosynthetic inhibitors on alkaloids and organogenesis in tobacco callus cultures [J]. Plant Cell Tissue Organ Cult,1987,9 (2):111-120.
  • 10陈子华.木本植物组织培养及其应用[M].北京:高等教育出版社,1986.81.

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