摘要
目的探讨乙型肝炎(下称乙肝)两对半与HBV-DNA含量的关系。方法392份血清用酶联免疫吸附试验(ELISA)法测定乙肝病毒(HBV)两对半,用FQ-PCR法检测HBV-DNA含量。结果乙肝两对半模式不同血清型的HBV-DNA阳性率不同,HBsAg(+)、HBeAg(+)、抗-HBc(+)最高,其HBV-DNA检出率为96.1%,对数均值为7.8±2.25;HBsAg(+)、抗-HBe(+)、抗-HBc(+)的HBV-DNA检出率为37.8%,对数均值为4.5±1.07;而HBsAg(+)、抗-HBc(+)的HBV-DNA的检出率为76.5%,对数均值为3.4±0.86。结论3种血清型的HBV-DNA检出率差异有统计学意义,并且拷贝数差异也有统计学意义。
Objective To investigate the correlation between HBV serum markers and HBV-DNA. Methods The HBV-DNA was detected by florescence quantitative PCR and HBV serum markers was detected by ELISA in 392 serum samples. Results In 198 of 206 cases with HBV surface antigen（＋）, HBV e antigen（＋ ）, anti-HBcAg antibody（ ＋ ） sample, fluorescence quantitative PCR results were positive, the positive rate was 96.1%, the logarithmic mean of HBV-DNA was 7.80±2.25. In 64 of 169 cases with HBV surface antigen（＋） ,anti-HBeAg antibody （＋） ,anti-HBcAg antibody（ ＋ ） sample, fluorescence quantitative PCR results were positive, the positive rate was 37.8 %, the logarithmic mean of HBV-DNA was 4.5 ± 1.07. In 65 of 85 cases with HBV surface antigen（ ＋ ）, HBV e antigen（＋ ）, anti-HBcAg antibody（ ＋ ） sample, fluorescence quantitative PCR results were positive, the positive rate was 76.5 %, the logarithmic mean of HBV-DNA was 3.40±0.86. Conclusion There is significant difference in HBV-DNA positive rate and copies in the three serum types.
出处
《检验医学与临床》
CAS
2007年第3期164-165,共2页
Laboratory Medicine and Clinic
