期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

青紫心甘薯黄酮减轻^(60)Co辐射诱导的小鼠胸腺淋巴细胞损伤的超微结构研究 被引量：3

Ultrastuctural study on administration of flavone from Ipomoea Batatas Poir.Cv.to reduce the ^(60)Co irradiation damage of murine thymic lymphocyte
在线阅读 下载PDF
导出
摘要 目的:观察青紫心甘薯黄酮对60Co辐射损伤体外培养的小鼠胸腺淋巴细胞超微结构的影响。方法:建立单次60Co辐射损伤体外培养的小鼠胸腺淋巴细胞的病理模型,应用透射电镜观察细胞超微结构的变化。结果:在强度为3Gy60Co辐射情况下,小鼠胸腺淋巴细胞超微结构在3 h后即出现核膜消失、核异染色质浓缩及边集、核碎裂、胞内空泡形成、线粒体嵴断裂、基质空泡化、粗面内质网扩张等典型细胞凋亡改变。2.500 g/L青紫心甘薯黄酮能抑制60Co辐射所致淋巴细胞的损伤,细胞的正常超微结构保存良好。结论:青紫心甘薯黄酮可有效地保护小鼠胸腺淋巴细胞对抗60Co的辐射损伤。 Objective： To investigate the role of flavone from lpomoea Batatas Poir. Cv. on ^60Co irradiation damaged normal murine thymic lymphocyte in vitro. Method： The model of ^60Co irradiation damaged murine thymic lymphocyte （radiantion intensity： 1. 176 × 10^-4J·cm^-2） was established. The ultrastructural changes of murine thymic lymphocyte were observed under the transmission electron microscope. Result： Ultrastructure of murine thymic lymphocyte was obviously changed 3h after ^60Co irradiation. Typical apoptosis appeared in ^60Co-irradiated murine thymic lymphocyte. Pretreated with flavone from lpomoea Batatas Jtoir. Cv. （2. 500 g/L） could effectively reduce the ultrastructural damage of murine thymic lymphocyte induced by ^60Co irradiation. Conclusion： tlavone from lpomoea Batatas Poir. Cv. may effectively protect murine thymic lymphocyte against ^60Co irradiation induced damage.
作者 侯颖一 朱莉 韩志武 李润生 王军强 王春波
机构地区 青岛大学医学院 青岛大学医学院附属医院 青岛大学青岛市农技站
出处 《电子显微学报》 CAS CSCD 2007年第1期64-68,共5页 Journal of Chinese Electron Microscopy Society
基金 省教育厅资助项目(编号:J04E17)
关键词 青紫心甘薯黄酮 ^60CO 小鼠胸腺淋巴细胞 超微结构 flavone from lpomoea Batatas Poir. Cv. ^60Co irradiation murine thymic lymphocyte ultrastructure
分类号 S531 [农业科学—作物学] R818.5 [医药卫生—放射医学]
  • 相关文献

参考文献15

  • 1Bodnarchuk I A. Analysis of the role of DNA repair. regulation of cell eycle and apoptosis in the radiationinduced adaptive response of mammalian cells[J]. Radiats Biol Radioecol,2003,43(1):19-28.
  • 2李家明,李丰.抗辐射药氨磷汀合成工艺的研究[J].安徽化工,2000,26(2):17-18. 被引量：8
  • 3Song J Y, Han S K, Bae K G. Radioprotective effects of ginsa, an immunomodulator[J]. Radiat Res, 2003,159(6):768-774.
  • 4Nishimura Y, Kim H S, Ikota N, et al. Radioprotective effect of chitosan in sub-lethally X-ray irradiated mice [J]. J Radiat Res,2003,44(1):53 - 58.
  • 5Kim S R, Jo S K, Kim S H. Modification of radiationresponse in mice by ginsenosides, active components of Panax glnseng[J]. In vivo,2003,17(1) :77 - 81.
  • 6Afaq F, Adhami V M,Ahmad N,Mukhtar H. Inhibition of ultraviolet B-mediated activation of nuclear factor kappa B in normal human epidermal keratinocytes by green tea vonstituent epigallocatechin-3-gallate[J]. Oncogene, 2003,22(7) : 1035 - 1044.
  • 7Sun Y, Mu Y, Li W, et al. Protection of epidermal cells against UVB injury by the anfioxidant selenium-containing single chain Fv catalytic antibody [J]. Acta Oncol, 2003,42(1) :4- 9.
  • 8姜平平,吕晓玲,姚秀玲,朱惠丽,张亮.紫心甘薯花色苷抗氧化活性体外实验研究[J].中国食品添加剂,2002,13(6):8-11. 被引量：112
  • 9Matsui T, Ebuchi S, Kobayashi M, et al. Anti-hyperglycemic effect of diacylated anthocyanin derived from ipomoea batatas cultivar ayamurasaki can be achieved through the alpha glucosidase inhibitory action[J]. J Agricul and Food Chemi,2002,50(25) :7244 - 7248.
  • 10Kawabe M, Tamano S, Aoki H, et al. Prevention by natural food anthocyanins, purple sweet potato color and red cabbage color, of 2-amino-l-methyl-6-phenylimidazo [4,5-b] pyridine(PhIP)-associated colorectal carcinogenesis in rats initiated with 1,2-dimethylhydrazine[J]. J Toxicol Sci,2002,27 (1): 57 - 68.

二级参考文献49

  • 1金鸣,蔡亚欣,李金荣,赵辉.邻二氮菲－Fe^（2＋）氧化法检测H_2O_2／Fe^（2＋）产生的羟自由基[J].生物化学与生物物理进展,1996,23(6):553-555. 被引量：570
  • 2胡野.细胞凋亡的分子医学[M].北京:军事医学科学出版社出版,2001.4-25.
  • 3Korycka-Dahl M,Richardson T.Photogeneration of suoeroxide anion in serum of bovine milk and in model systems containing riboflavin and amino acid[J]. J Dairy Science,1977,61:400.
  • 4Yao RY, Wang CB.Protective effect of polypeptide from Chlamys farreri on HeLa cells damaged by ultraviolet A[J]. Acta Pharmacol Sin, 2002,23:1018.
  • 5Klassen NV,Walker PR,Ross CK,et al.Two-stage cell shrinkage and OER for adiation-induced formation of apoptotic bodies in rat thymus[J].Radiat Res,1985,101:123.
  • 6Wyllie AH,Morris RG,Smith AL,et al. Chromatin cleavage in apoptotic with condensed chromatin morphology and dependence on macromolecular synthesis[J].J Pathol,1984,142:67.
  • 7Zhou LJ,Song W,Zhu XZ,et al.Protective effects of bilobalid on amyloid beta-peptide 25-35-induced PC12 cell cytotoxicity[J].Acta Pharmacol Sin,2000,21(1):75
  • 8Shang YZ,Ye JM,Tang XC.Improving effects of huperzine A on abnormal lipid peroxidation and superoxide dismutase in aged rats[J].Acta Pharmocol Sin,1999,20(9):824.
  • 9Zweier JL,Kuppusamy P,Williams R,et al.Measurement and characterization of postischemic free radical generation in the isolated perfused heart[J].J Biol Chem,1989,18890.
  • 10Ohkawa H,Ohishi N,Yagi K.Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction[J].Anal Biochem,1979,95:351.

共引文献133

同被引文献84

引证文献3

二级引证文献20

电子显微学报
2007年 第1期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部