摘要
目的考察乙醇回流提取法提取黄连中盐酸小檗碱时主要影响因素。方法通过正交实验法确立试验体系,采用荧光分析法测定盐酸小檗碱含量。结果选择不同的提取条件盐酸小檗碱提取率有显著差异。结论最优提取工艺是:50%乙醇溶液、8倍体积、85℃回流提取2次,每次2 h。
Objective To study the major influential factors of extraction by alcohol flowing on berberine hydrochloride in Coptis Chinensis Franch. Methods To establish the experiment system by orthogonal experimental design and adopt fluorometry to determine the content of berberine hydrochloride. Results We observed the significant difference of the extraction rate of berberine hydrochloride under the different extraction conditions. Conclusion The optimum extraction process was achieved： the addition of 50% alcohol（ 8 times raw herb in volume） into materials, refluxing and extracting for 2 times at 85℃.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2007年第2期437-438,共2页
Lishizhen Medicine and Materia Medica Research
关键词
黄连
盐酸小檗碱
提取工艺
正交实验设计
荧光分析法
Coptis Chinensis Franch.
Berberine Hydrochloride
Extraction process
Orthogonal experimental design
Fluorometry
