摘要
目的研究依普拉芬对大鼠成骨细胞一氧化氮生成以及内皮型一氧化氮合酶（eNOS）mRNA表达的影响，探讨其治疗骨质疏松的细胞作用机制。方法体外分离培养新生SD大鼠颅盖骨成骨细胞，取第2代细胞，培养液中分别加入不同浓度的依普拉芬，72h后，测定各组细胞培养液中的NO浓度，RT—PCR方法检测各组细胞中eNOSmRNA的表达。结果与阴性对照组相比，各浓度组的依普拉芬均可增加成骨细胞培养液中的NO浓度并促进eNOSmRNA的表达（均P〈0．01）。其中10^-8～10^-5mol／L浓度之间作用最为显著。结论一定浓度的依普拉芬可以增加成骨细胞NO合成，但进eNOSmRNA的表达，从而发挥其促进成骨作用。
Objective To study the effect of Ipriflavone （7-isopropoxyisoflavone, IP） on nitric oxide （NO） synthesis and expression of endothelium nitric oxide synthase （eNOS） mRNA in cultured rat osteoblasts in vitro. Methods New born rat calvarid osteoblastic cells were isolated and cultured with the medium containing IP at different concentrations. After 72 h, the levels of NO in the media were detected and the mRNA expression of eNOS assayed by reverse-transcriptase polymerase chain reaction （RT-PCR）. Results In comparison to controls, IP could increase the concentration of NO in the media and the expression levels of eNOS mRNA （all P〈0.01）. The effects of 10^-8～10^-5mol/L IP were most significant. Conclusion IP could significantly increase the synthesis of NO and the expression levels of eNOS mRNA in cultured rat osteoblasts in vitro. Thus it could promote the proliferation and differentiation of cultured rat osteoblasts in vitro.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2007年第1期71-74,共4页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
