摘要
目的研究银杏叶提取物(GBE)对哮喘豚鼠气道上皮E-钙黏素(E-cadherin)水平的影响,以探讨银杏叶提取物治疗哮喘的机制。方法将32只豚鼠随机分成正常对照组(A组)、哮喘未治疗组(B组)、哮喘地塞米松治疗组(C组)、哮喘GBE治疗组(D组)。通过苏木精-伊红染色观察气道壁嗜酸性粒细胞(EOS)浸润情况,利用免疫组织化学检测气道上皮的E-钙黏素蛋白水平,利用Western blot检测支气管肺泡灌洗液(BALF)中可溶性E-钙黏素胞外段的水平,利用RT-PCR检测气道上皮细胞内E-钙黏素mRNA水平。结果C组、D组气道上皮的E-钙黏素水平明显高于A组,低于B组;C组、D组气道壁EOS计数及BALF中E-钙黏素胞外段的水平则显著高于A组,低于B组;各组上皮细胞内E-钙黏素mRNA水平无显著性差异。结论GBE可抑制气道上皮E-钙黏素的胞外段裂解、脱落,从而维持E-钙黏素水平,保护上皮细胞的屏障功能,减轻气道炎症。这很可能是GBE治疗哮喘的机制之一。
Objective To explore the therapeutic mechanisms of Ginkgo biloba extract （GBE） by studying the effect of GBE on the E-cadherin in airway epithelia of asthmatic guinea pigs. Methods Thirty-two guinea pigs were randomly divided into 4 groups; control group （group A）, asthmatic group （group B）, dexamesone-treated asthmatic group （group C） and GBE- treated asthmatic group （group D）. Eosinophilic inflammation of airway wall was observed with hematoxylin-eosin （HE） staining. The levels of E-cadherin mRNA and protein in airway epithelia were detected by semi-quantitative RT-PCR and immunohistochemical staining respectively, and the soluble extracellular portion of E-cadherin in BALF by Western blot. Results The level of E-cadherin in group C and group D was higher than that in group A and lower than that in group B; The number of EOS in the airway wall and the amount of soluble extracellular portion of E-cadherin in BALF were significantly more than that in group A and lower than that in group B. There was no significan difference in E-cadherin mRNA in airway epithelia among four groups. Conclusion The cleavage and shedding of the extracellular portion of E-cadherin were inhibited by the treatment of GBE, which could maintain the levels of E-cadherin, ameliorate the inflammation of airway and protect the normal function of the airway epithelia barrier.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2007年第1期41-44,共4页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
