摘要
利用随机引物扩增多态性DNA(RAPD)技术,对落羽杉属原种及其杂交后代共13个样本进行亲缘关系鉴定。从100个10bp的随机引物中筛选出13个扩增效果较好的引物,共扩增出92条带,其中72条为多态性带。根据扩增结果进行聚类分析,结果表明杂交F1代中山杉302(落羽杉×墨西哥落羽杉)与母本落羽杉更为接近。在回交后代(中山杉302×墨西哥落羽杉)中,BCF1118与BCF1140相似系数最大;BCF11与母本F1302亲缘关系最近;BCF1118与父本墨西哥落羽杉亲缘关系最近。
The genetic relationship of thirteen species and hybrids of Taxodium was analyzed by random amplified polymorphic DNA（RAPD）. Thirteen better primers were selected from one hundred arbitrary 10 bp primers. Total 92 bands were produced, with which 72 were polymorphic. According to the cluster analysis, the results showed that the genetic relationship of first crossed generation （ F1 ） Taxodium ‘Zhongshansha 302＇ （ T. distichum × T. mucronatum） was closer to the female parent T. distichum. Among the hybrids of first backcrossed generations（ BCF1 ）, the genetic similarity coefficients of RAPD between BCF1118 and BCF1140 was the highest, and BCF11 was closer to the female parent Taxodium ‘Zhongshansha 302＇, while BCF1118 was closer to the male parent T. mucronatum.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2007年第2期48-51,共4页
Scientia Silvae Sinicae
基金
国家科技部农业科技成果转化资金项目(03EFN213200101)
江苏省"六大人才高峰"项目(06-G-039)
