摘要
目的研究新型纳米羟磷灰石/聚酰胺66(nHA-PA66)对龋性牙本质中常见致病菌的体外抗菌作用。方法采用变形链球菌、粘性放线菌和干酪乳杆菌的标准株,通过琼脂扩散法比较nHA-PA66固化膜片、nHA-PA66糊剂、nHA-PA66碘仿糊剂、羟磷灰石糊剂及氢氧化钙糊剂对3种实验菌的抑菌环直径大小。结果nHA-PA66固化膜片与nHA-PA66糊剂对变形链球菌无抗菌作用,对粘性放线菌和干酪乳杆菌有极轻微抗菌作用,nHA-PA66固化膜片与nHA-PA66糊剂之间抑菌环直径差异无统计学意义(P>0.05);nHA-PA66碘仿糊剂的抗菌能力增强,但与氢氧化钙糊剂相比,二者间差异无统计学意义(P>0.05);羟磷灰石糊剂对3种实验菌均无抗菌作用。结论nHA-PA66作为一种新型纳米生物材料,单独使用时对变形链球菌、粘性放线菌和干酪乳杆菌几乎不具有抗菌性能,但可通过与碘仿的联合应用来提高nHA-PA66用作盖髓材料时的抗菌能力。
Objective To investigate the in vitro antimicrobial activity of nano-hydroxyapatite/polyamide 66 composites （nHA-PA66） as a pulp capping agent. Methods The micro-organisims used in this study included Streptococcous mutans （S.mwtans）, Lactobacillus casei （L.casei） and Actinomyces viscosus （A.viscosus）, three standard bacterial strains predominant in deep carious lesions. Agar diffusion test was used to determine the diameter of bacterial inhibition zones of nHA-PA66 compared with hydroxyapatite paste, calcium hydroxide paste and nHA-PA66 mixed with iodoform paste. Results It showed that the polymerized films and fresh paste of nHA-PA66 had no antimicrobial activity to S.mutans and very little to L.casei and A.viscosus. The antimicrobial activity of nHA-PA66 mixed with iodoform paste obviously increased, but no significant difference compared with calcium hydroxide paste. There. was no antimicrobial activity of hydroxyapatite paste to the three test bacterial strains. Conclusion These findings suggest nHAPA66 used as pulp capping agent alone almost has no antimicrobial effect to the three test bacteria, but the antimicrobial activity can be increased by combining with iodoform.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2007年第1期26-28,共3页
West China Journal of Stomatology
基金
四川省科委重点资助项目(02SG022-001)
关键词
纳米羟磷灰石/聚酰胺66
氢氧化钙
碘仿
抗菌作用
nano-hydroxyapatite/polyamide 66
calcium hydroxide： iodoform
antimicrobial activity
