摘要
在含有不同浓度秋水仙素双层培养基上以叶片作外植体诱导毛泡桐同源四倍体植株,用变异植株根尖细胞染色体计数和成熟叶片中单细胞DNA含量测定的方法进行倍性分析。结果表明,在16个试验组合中,预培养6d的毛泡桐叶片经20mg·L-1秋水仙素处理72h的四倍体诱导率可达20%。
Induction of autotetraploid of Paulownia tomentosa from the leaves of the diploid with different concentrations of colchicines on the double MS media through chromosome numbering of the plant tip cells and DNA content in leaf Cell were investigated. The results indicated that the highest tetraploid induction rate might reach 20% from the leaves pre-cultured for 6 d and treated with 20 mg·L^-1 colchicine for 72 h on the double media among 16 experiment combinations.
出处
《植物生理学通讯》
CSCD
北大核心
2007年第1期109-111,共3页
Plant Physiology Communications
基金
河南省高校创新人才基金(2002012)。
关键词
毛泡桐
叶片
秋水仙素
四倍体
培养基
Paulownia tomentosa
leaf
colchicine
tetraploid
medium
