摘要
用10-2mol/L油菜素内酯浸泡绿豆种子,其幼苗下胚轴插条培养2d后,不定根长度、根数和生根范围均增加,分别比对照增加12.5%,27.4%和37.6%,插条基部细胞的吲哚丙酮酸脱羧酶、吲哚乙醛氧化酶和色氨酸转氨酶的比活性分别比对照提高120.6%,82.1%和54.7%,吲哚乙酸含量增加22.3%,插条基部细胞进入细胞周期S期的数目增加23%。结果表明,BR可能通过增加IAA含量,促进DNA合成和细胞分裂,从而促进不定根的发生。
The pre-treatment of germinating seeds of mung bean with 10^-2 mol/L of brassinolide （BR） is found to help the advititious root formation and root growth in hypocotyl cuttings of mung bean. Root length, root numbers and rooting areas per cutting were increased by 12.5%, 27.4%, 37.6%, respectively, over the controls after two days of treatment. Enzyme activities of indole pyruvate decarboxylase, indole acetaldehyde oxidase and tryptophan aminotransferase were increased by 120.6%, 82.1% and 54.7%, respectively, and indole acetic acid （IAA） content was increased by 22.3% in the rooting zone of the cuttings. Cell numbers in S phase in the basal part of rooting zone was increased by 23% in the BR treatment. Results indicate that BR helps the rooting by increasing IAA contents
出处
《科技导报》
CAS
CSCD
2007年第5期56-60,共5页
Science & Technology Review
关键词
油菜素内酯
绿豆
不定根
吲哚乙酸
细胞周期
brassinolide
mung bean
adventitious rooting
indole acetic acid
cell cycle
