摘要
目的探讨新疆枸杞子的抗氧化作用。方法提取大鼠肝微粒体,制备Fe2+-半胱氨酸诱发的肝微粒体脂质过氧化损伤的体外模型,检测新疆枸杞子的20个样品在不同终反应浓度(100μg/ml、50μg/ml、10μg/ml、1μg/ml)时对丙二醛(MDA)含量变化的影响,观察样品清除活性氧的抗氧化作用。每个浓度重复实验3次,最后得出样品在每个浓度的MDA抑制率的平均值。结果12号样品即大孔树脂柱95%乙醇洗脱部分的MDA抑制率>50%,得出3组IC50值,分别为37.8%、23.1%、33.1%,平均为31.3%。结论新疆枸杞子的大孔树脂柱95%乙醇洗脱部分具有抗氧化作用。
Objective： To investigate the antioxidation action of Fructus Lycii in Xinjiang. Methods： Sprague Dawley rats were killed and then the livers were removed to isolate the microsome which can generate the reactive oxygen species in the presence of Fe^2＋-Cysteine. In these peroxidative damage models, at different final concentrations （100μg/ml.50 μg/ml. 10 μg/ml. 1μg/ml）, the contents of malondialdehyde （MDA） were examined for analyzing the antioxidative action of Fructus Lycii in Xinjiang. At each concen- tration, we tested three times, at last, we got the average inhibition ratio of MDA. Result： The inhibition ratio of MDA of the number twelfth sample which was 95 % alcohol elutropic fraction of macroporous resin ofFructus Lycii was more than 50%. We got IC50. They were 37.8%, 23.1% and 33.1% respectively. The average IC50 was 31.3%. Conclusion： The results have indicated that 95% alcohol etutropic fraction of macroporous resin of Fructus Lycii in Xinjiang shows antioxidation.
出处
《新疆医科大学学报》
CAS
2007年第1期40-42,共3页
Journal of Xinjiang Medical University
基金
自治区高校科研计划创新研究群体基金资助(XJEDU2004G08)
关键词
新疆枸杞子
微粒体
丙二醛
抗氧化
Fructus Lycii in Xinjiang
microsome
malondialdehyde（MDA）
antioxidation
