摘要
目的探讨依达拉奉在失血性休克中,对肠组织细胞间粘附分子-1及肠屏障功能的影响。方法新西兰大白兔30只,随机分为失血性休克组、依达拉奉处理组和正常对照组。失血性休克采用股动脉放血制作模型,休克持续2h后回输失血及等量林格液复苏;依达拉奉组在复苏时静注1次依达拉奉5mg/kg;对照组不行放血处理。各组复苏后2h,取小肠组织行常规病理学检查,并制备肠组织匀浆,采用ELISA法测定细胞间黏附分子-1(ICAM-1);检测肠组织匀浆液髓过氧化物酶(MPO)活性;留取血浆检测D-乳酸水平。结果与失血性休克组比较,依达拉奉处理组小肠组织中ICAM-1及MPO均降低,肠黏膜损伤程度轻,血浆D-乳酸水平也低。结论早期大剂量运用依达拉奉,能够抑制失血性休克后肠组织ICAM-1的表达。减轻肠结构的破坏,保护肠黏膜屏障功能。
Objective To investigate the influence of edaravone on intestinal tissue ICAM - 1 in intestinal barrier dysfunction after hemorrhagic shock of rabbits. Methods Thirty rabbits were randomly divided into three groups ： control groups, hemorrhagic shock group edaravone - treatment group. Hemorrhagic shock was induced by modified Wiggers method until mean arterial pressure was stabilized within the range of 35 to 40 mm Hg. The hypotension was maintained for 2 hours before lost blood and equivalent amount of Ringer＇ S solution were infused. 5 mg/kg of edaravone was given when edaravone treatment group began to resuscitate. At 60 min after reperfusion, rabbits were decapitated. The levels of ICAM - 1 and the activity of MPO in intestine homogenates - were determined. At the same time, the content of D - lactate in the plasma Was determined and pathological changes in intestinal were observed. Results Compared with control group, ICAM - 1, MPO and D - lactate were significantly increased in hemorrhagic shock group and they reversed to some degrees with the treatment of edaravone. The histopathologic results were consistent with the biochemical markers. Conclusion Edaravone can inhibit the expression of ICAM- 1 in intestinal tissue and has potential protective effects on the intestinal barrier function after hemorrhagic shock.
出处
《中国急救医学》
CAS
CSCD
北大核心
2007年第3期243-245,共3页
Chinese Journal of Critical Care Medicine
基金
河北省卫生厅医学研究重点课题(No05036)
