摘要
目的寻找一种较为理想的诱导人骨髓间充质干细胞分化为神经元的诱导剂及方法。方法采用人淋巴细胞分离液从骨髓中分离出骨髓间充质干细胞,体外培养、扩增后,以不同组合的复合神经诱导剂加以诱导,诱导期间观察细胞形态的变化,通过免疫细胞化学鉴定诱导细胞的分化情况,并用SPSS12.0进行数据分析。结果人骨髓间充质干细胞在加入不同组合的复合神经诱导剂后,均出现了胞体收缩、突起伸出的神经元样改变。免疫组化结果显示,兔抗人巢蛋白(nestin)、鼠抗人神经丝单克隆抗体(neurofilament,NF)出现了不同的阳性率,其中以E组阳性率最高,nestin(5.653±1.228)%,NF(74.724±3.651)%,兔抗人胶质纤维酸性蛋白(gliafiber acid protein,GFAP)免疫组化染色均呈阴性,SPSS12.0分析显示各诱导组之间差异具有显著性意义(P<0.05)。结论本实验探索出一种较为理想的诱导人骨髓间充质干细胞分化为神经元的复合神经诱导剂及方法。
Objective To find a relatively ideal revulsant and method of differentiation of human bone mesenchymal stem cells into neurons. Methods The human bone mesenchymal stem cells, which were isolated from bone marrow with the lymphocyte separate liquid, cultured and expanded in vitro, were induced by different combined neuro - revulsant. The morphologic change of cells was being observed during inducing, period, the differentiation of induced cells was identified by immunocytochemistry, and data was analyzed by SPSS 12.0. Results Human bone mesenchymal stem calls, which were induced by different combined neuro-revulsant, exhibited neuronal morphological characteristics, cellular shrinkage and neurite growth. The result of immunocytochemistry showed that the positive rates of nestin and neurofilament （NF） of different groups were dissimilar, and the group E had the tiptop positive rate in all groups, nestin （5.653 ±1.228）% and NF （74.724 ± 3.651 ）%. The results of gliafiber acid protein immunocytochemistry stain were negative in all groups. The results of data analysis by SPSS 12.0 showed that the difference between the different induced groups had prominence signification. Conclusion A relatively ideal combined neuro - revulsant and method of inducement of human bone mesenchymal stem cells into neurons were found.
出处
《中国骨与关节损伤杂志》
2007年第2期119-121,共3页
Chinese Journal of Bone and Joint Injury
关键词
骨髓间充质干细胞
诱导
神经元
Human bone mesenehymal stem ceils
Inducement
Neuron
