摘要
结合计算机技术和生物信息学的方法,采用组合的信号肽分析软件SignalPv3.0、TargetPv1.1、Big-PIpredictor、TMHMMv2.0和SecretomeP对已公布的1486个稻瘟菌(magnaporthegrisea)小蛋白基因的N-端氨基酸序列进行信号肽分析,同时系统分析了信号肽的类型及结构。分析结果表明,在1486个稻瘟病菌小蛋白中,119个具有N-端信号肽的典型分泌蛋白。其中116个具有分泌型信号肽,1个具RR-motif型信号肽,2个具信号肽酶II型信号肽。在稻瘟病菌基因组中,分泌型小蛋白的序列是高度趋异的,仅出现少数氨基酸组成完全一致的信号肽,为进一步确认具有相同信号肽的分泌蛋白是否具有同源性,分别用BLAST2SEQUENCES对具有相同信号肽的分泌蛋白进行了序列对比。结果表明,具有相同信号肽的分泌蛋白同源性非常高。同时还采用Sublocv1.0对1486个小蛋白的亚细胞位置进行了预测,结果显示小蛋白的可能功能场所包括细胞质、细胞外、线立体和细胞核,功能场所位于细胞核的小蛋白是最多的。
The internet-based software such as the SignalP v3.0, TargetP v1. 1, Big-PI predictor ,TMHMM v2.0 and SecretomeP were combined to predict the signal peptides, and the signal peptide-dependent secreted short proteins among the 1 486 ORFs in magnaporthe grisea genome. The result showed that total 119 short proteins were the secreted ones containing signal peptides among 1 486 short proteins, and 116 were secretary types, 1 was RR-motif types, 2 were Signal- Pase Ⅱ types. There were few of the same signal peptides occurred in the secreted short proteins of magnaporthe grisea genome, in order to compare the hhomology among the secreted short proteins with the same signal peptides. The BLAST 2 SEQUENCES is used to align the two protein sequences. The alignment result indicated that homo;ogy of these sequences with the same signal peptide was very highly conservative in amino acid of complete gene. The Subloc v1. 0 is used to analysis the subcellular localization of the 1486 short proteins in magnaporthe grisea genome. The result showed that their sublocalization were cytoplasmic, extracellular, mitoehondrial and nuclear, most of them were in nuclear.
出处
《生物技术通报》
CAS
CSCD
2006年第C00期276-282,共7页
Biotechnology Bulletin
基金
国家973计划项目(2006CB100203)
国家自然科学基金项目(30360061)
关键词
稻瘟病菌
小蛋白
信号肽
分泌
Magnaporthe grisea Small protein Signal peptide Secretion
