摘要
The spectrum of antimicrobial effects of melittin were investigated on 19 pathogens by using a cylin-der-plate method with serial dilutions. Bacteriostatic efficiency and possible mechanistic effects were monitored via growth curves. The mechanism of inhibition was further analyzed by SDS-PAGE, flow cytometry and electron microscopy. Melittin had a wide inhibition spectrum and killed pathogens ef-fectively, and bacteriostatic action was influenced by factors such as pH and temperature. We eluci-dated three inhibitory mechanisms: melittin integrated with the cell membrane causing cell bursting and cytoplasm release, inhibited the synthesis of proteins and caused the cytoplasm to condense, and delayed pathogens in phase I (or phase G1) so that they could not complete the cell cycle. These re-sults suggest that melittin could serve as a broad-spectrum biological pesticide with fast-action and high-efficiency.
The spectrum of antimicrobial effects of melittin were investigated on 19 pathogens by using a cylinder-plate method with serial dilutions. BacteriostaUc efficiency and possible mechanistic effects were monitored via growth curves. The mechanism of inhibition was further analyzed by SDS-PAGE, flow cytometry and electron microscopy. MelitUn had a wide inhibition spectrum and killed pathogens effectively, and bacteriostatic action was influenced by factors such as pH and temperature. We eluci- dated three inhibitory mechanisms： melitUn integrated with the cell membrane causing cell bursting and cytoplasm release, inhibited the synthesis of proteins and caused the cytoplasm to condense, and delayed pathogens in phase Ⅰ（or phase G1） so that they could not complete the cell cycle. These resuits suggest that melittin could serve as a broad-spectrum biological pesticide with fast-action and high-efficiency.
基金
Supported by the National Natural Science Foundation of China (Grant No. 30170112)
the International Cooperation Project (Grant No. 30310103130)
