摘要
本研究以枯草杆菌肌苷产生菌ＳＤ９４０１（Ａｄｅˉ＋Ｔｈｉˉ＋Ｈｉｓˉ＋８—ＡＧｒ＋６－ＭＰｒ）为出发菌株，经紫外线和硫酸二乙酯诱变，在以肌苷为唯一碳源的补充培养基上筛选到一株核苷水解酶缺失菌株。实验结果表明这一突变株积累比亲株高３０％左右的肌苷，平均达到２６．８９ｍｇ／ｍｌ，最高到２８．０３ｍｇ／ｍｌ。且发酵液中未测出次黄嘌呤。
The paper reported an inosine-producing auxotroph of a Bacillus strain SD9401 (Adeˉ+Thiˉ+ Hisˉ+ 8-AGr+ 6-MPr),which was mutanted by U.V and Diethyl Sulfate,was isolated on the medium containing Inosine as Sole energy source.We got a strain failed to grow on this medium and it assumed to be deficient in nucleoside-hydrolyzing activity.The research results showed that the mutant was found to accumulate Inosine 30 persant more than the parent strain.It reached an average of 26.89mg/ml,maximal of 28.03mg/ml, FUrther,No hypoxanthine was found in this Culture fluid.
出处
《生物技术》
CAS
CSCD
1996年第5期28-30,共3页
Biotechnology
关键词
肌苷
核苷水解酶
发酵
Inosine
Nucleoside-hydrolyzing
Mutant
Hypoxanthine
