摘要
研究了采用海藻酸钠固定化克雷伯氏菌降低发酵液中的残留甘油浓度。与游离细胞相比,固定化细胞有利于固液分离,且对甘油浓度的降低效果也略好。培养16 h的发酵液中残留甘油浓度降低了91%。考察了固定化条件对降低残留甘油浓度的影响,确定了固定化最佳条件为:菌体∶Na-alginate=1∶10(质量体积比),3%Na-alginate,4%CaCl2,固定化24 h;固定化处理后可以提高发酵液中的1,3-丙二醇浓度。室温培养固定化细胞的实验效果与37℃培养相差不大。动态培养固定化细胞38 h,残留甘油浓度降低了82.4%,固定化细胞可以重复使用。通过固定化细胞处理可以减小甘油对蒸馏1,3-丙二醇时产生的影响,从而提高1,3-丙二醇的纯度和收率。
Removing residual glycerol in flocculated fermentation broth by immobilized Klebsiella pneumoniae with Na-alginate bead was investigated. The efficiency of immobilized cell was better than free cell and the concentration of residual glycerol was reduced by 91% after 16 h. The influence of immobilized conditions on removing residual glycerol was studied. The optimum conditions were that the ratio of biomass to Na-algihate was 1 ： 10, the mass fraction of Na-alginate was 3%, the mass fraction of CaCl2 was 4% and the time of immobilization was 24 h. The 1,3-propanediol concentration was increased by using immobilized cell. The effect of glycerol removal using cultivating immobilized cell at room temperature had no significant difference with that at 37℃. When cultivating using continuous glass column bioreactor, the concentration of residual glycerol was reduced by 82.4% after 38 h. The immobilized cell could be reused. The influence of glycerol on distillation by using immobilized cell was reduced. The purity and yield of 1,3-propanediol were increased.
出处
《化学与生物工程》
CAS
2007年第2期37-40,共4页
Chemistry & Bioengineering
基金
国家自然科学基金资助项目(20325622
20576013
50373003)
博士点基金资助项目(20030010004)
国家"十五"攻关计划资助项目(2004BA411B05)
关键词
甘油
1
3-丙二醇
固定化
海藻酸钠
克雷伯氏菌
glycerol
1,3-propanediol
immobilization： Na-alginate
Klebsiella pneumoniae
