摘要
目的探讨丰富环境刺激及水疗对新生大鼠缺氧缺血性脑损伤(hypoxia-ischemia brain damage,HIBD)的干预效应及可能机制。方法新生7d龄SD大鼠分为干预组、非干预组及假手术组,干预组又分为丰富环境组、水疗组、丰富环境+水疗组。干预组在HIBD第2天分别予相应干预治疗,持续28d。以免疫组织化学法检测不同时相点海马CA1区NMDA受体NR1亚单位的表达,并于干预结束后利用Morris水迷宫测试各组学习记忆能力,透射电镜观察海马突触结构。结果干预28d,干预组海马CA1区NR1的蛋白表达及学习记忆能力明显高于非干预组,其中丰富环境组、丰富环境+水疗组与假手术组比较无显著性差异(P>0.05),水疗组仍明显低于假手术组(P<0.05)。透射电镜下,干预组大鼠海马突触后膜致密物(postsynaptic density,PSD)较多,非干预组海马PSD最少,结构疏松。结论丰富环境刺激及水疗有助于新生大鼠HIBD的恢复,其中以丰富环境刺激、丰富环境刺激联合水疗干预效果显著,海马NMDA受体表达变化是其可能的影响机制之一。
Objective To investigate the effects of enriched environment and hydrotherapy on neonatal rats with hypoxia-ischemia brain damage. Methods The 7-day-old SD rats were randomly divided into intervention group （ enriched environment group, hydrotherapy group, enriched environment ＋ hydrotherapy group）, non-intervention group and sham operation group. The intervention group were treated by the methods mentioned above respectively for 28 days after HIBD. Immunohistochemical method was used to detect the expression of NMDA receptor NR1 subunit in hippocampal CA1 region at different time points. Behavior training （ Morris water maze） for the learning and memory abilities and electron microscopy for synaptic structure were performed. Results The expression of NR1 in hippocampal CA1 region increased and the learning and memory abilities were improved obviously in intervention group as co difference between the enriched environment group, mpared with non-intervention group, but showed no marked enriched environment ＋ hydrotherapy group and sham group （P 〉 0. 05 ）, reduced obviously in hydrotherapy group as compared with sham group. Postsynaptic density （PSD） in hippocampus was more in intervention group than non-intervention group. Conclusion Enriched environment stimulus and hydrotherapy can promote the rehabilitation of neonatal rats with HIBD. The intervention effects of enriched environment and combined application of enriched environment stimulus and hydrotherapy is better than hydrotherapy. The change of NMDA receptor in hippocampus might be one of the mechanism.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2007年第6期534-537,共4页
Journal of Third Military Medical University
关键词
脑缺血
脑缺氧
NMDA受体
学习记忆
丰富环境
水疗
cerebral ischemia
cerebral hypoxia
NMDA receptor
learning and memory
enriched environment
hydrotherapy
