摘要
胃肠道粘膜层弥漫分布着大量内分泌细胞，产生多种胃肠道激素，对胃肠道许多良恶性疾病的发生与发展起着十分重要的作用。本文应用免疫组织化学技术，对慢性萎缩性胃炎、肠化生、异型增生和胃窦癌病人胃窦粘膜胃泌素细胞（Ｇ细胞）、Ｄ细胞和５－羟色胺（５－ＨＴ）免疫反应细胞进行了检测，结果如下：一、双重染色后，Ｇ细胞胞质呈红色，Ｄ细胞呈黑色，带状分布于粘膜中下１／３处，细胞核均为阴性。细胞形态多样，以棱形、锥体形和不规则形为主。有的细胞有突起，伸入到其它腺细胞之间或基膜之间，有的Ｄ细胞突起达到或朝向腺腔。胞质及突起内分泌颗粒多少不等，深浅不一。随着萎缩及肠化生的加重，分泌颗粒越来越少，着色越来越浅。两种细胞计数也逐渐下降，与正常对照组比较具有显著性差异。（Ｐ＜０．０５－０．０１）。在癌旁组织观察，两种细胞计数最低，肠化区Ｇ细胞缺如。二、５－ＨＴ免疫阳性细胞散在分布于幽门腺中下部，形态各异，以卵圆形、锥体形和不规则形为主。分散于胞质的反应颗粒呈红色，胞核阴性。有的细胞有细长突起，伸入到管腔或与相邻细胞接触。各组５－ＨＴ阳性细胞对比，从正常到萎缩及萎缩伴肠化改变中，细胞数目逐渐下降，但在癌旁粘膜中此细胞数目增加，与重度萎缩伴重度?
There are numerous endocrine cells in gastroenteric mucosa.These cells produce many gastroenteric hormones that make importation to occurrence and development of gastroenteric benign or malignant diseases.To investigate the relationship of gastrin,somatostatin,5-Hydroxytryptamine and pathologic changes in chronic atrophic gastricis(CAG),intestinal metaplasia ( IM) , dysplasia(dys) and gastric carcinoma, human gastric antral mucosa of 146 cases from above diseases were observed with immunohistochemical technique.The results were shown as follows:1.After double staining,the cytoplasms of G cell and D cell were divided into red and black color.Nuclei had no color. They were located in middle -lower zone of the mucosal glands.Both kinds of cell were different in shapes.shown as ovoid , pyranidal and irregrlar ones.The protrusion Could be found in some cells and they projected between otherglandulouscells or basal membrane.Top or protrusion of some cells ended glandulous cavitys,More or less endocrine granules were found in cytoplasm and protrusion.with atrophic aggravating, endocrine granules became less and less and staining color was thinner and thinner. Cell count of both kinds was declined and shown significant difference from that of the control group(P<0.05-0.01).Cell count was the least in mucomembrane nearby carcinomas G cell was not found in intestinal metaplasia region.2 . The 5-HT immunoreactive cells located in middle-lower zone of pyiom , gland Their shapes were shown in ovoid,pyranidal and imegulas one mainly.Immunoreactive granules diffused in cytoplasm and appeared as red color. Nuclei were negative. Protrusion of some cells were observed and they arrived glandulous cavity or adjacent cell. Cell count of each groupwascontrasted from normal to atrophy and atrophy accompanied intestinal metaplasia.The count was decreased gradually.But,in mucomembrane nearby carcinomas,the number was increased and shown significant difference form that of the severe atrophy accompanied severe intestinal metaplasia(P< 0.05).3 .To sum up,G cell D cell and 5-HT immunoreactive cells were seriously harmed in chronic atrophic gastritis and intestinal metaplasia, but they were not obviously affected in dysplasia.Fuctions of cavitary secretion and side-secretion was existed in G,D and 5-HT immunoreactive cells.They could coexist in one cell between G and D or 5-HT and D cell.To gastric carcinoma,action of D cell was not lear.howerer,G and 5-HT immunoreactive cells may promote growth and expansion of gastric carcinoma.
出处
《胃肠病学和肝病学杂志》
CAS
1996年第4期270-275,共6页
Chinese Journal of Gastroenterology and Hepatology
基金
河北省教委自然科学基金
关键词
慢性
萎缩性胃炎
胃泌素
生长抑素
血清素
Gastrin Somatostatin 5-Hydroxytryptamine Chronic atrophic gastritis
