摘要
目的 观察罗格列酮和二甲双胍对大鼠非酒精性脂肪肝病（NAFLD）的预防作用，并探讨其可能作用机理。方法 32只雄性SD大鼠分为4组：正常对照组、NAFLD对照组、罗格列酮干预组、二甲双胍干预组。以高脂饲料饲养诱发脂肪肝大鼠模型。12周末，处死所有动物，检测血脂、肝功能、肝脂质含量、肝组织病理学等，并检测血肿瘤坏死因子α（TNF-α）及肝组织TNF-α、肉毒碱软脂酰转移酶Ⅰ（CPT—Ⅰ）、乙酰辅酶A羧化酶（ACC）、脂肪酸合成酶（FAS）的mRNA表达等指标。结果 与NAFLD对照组比较，罗格列酮干预组及二甲双胍干预组的血TG（0．55±0．08，0．71±0．15 vs 0．85±0．15）mmol／L、ALT（49．1±7．4，56．0±33．6vs110．6±44．2）U／L及肝组织TG含量（33．7±8．2，37．2±7．8vs54．8±7．6）mg／g均显著下降（P均〈0．05），其肝脂肪变性及炎症程度亦显著减轻，其血清TNF-α（187．5±20．3，211．9±26．6vs324．2±34．2）pg／ml及肝组织TNF-α（0．35±0．12，0．49±0．18vs0．85±0．20）、FAS mRNA表达水平（0．34±0．13，0．45±0．15vs0．68±0．19）亦显著下降（P均〈0．05）。结论 罗格列酮和二甲双胍，可对高脂饲料饲养诱发的大鼠NAFLD产生保护作用；其保护作用与减少TNF-α和FAS表达有关。
Objective To investigate the preventive effects of rosiglitazone and metformin on nonalcoholic fatty liver disease （NAFLD） in rats, and explore the possible mechanisms involved. Methods Thirty-two male SD rats were randomized into normal control group, NAFLD control group, rosiglitazone-prevention and metformin-prevention groups. NAFLD rat model was established by feeding with high caloric diet. Serum TC, TG, ALT, AST, TNF-α, and liver lipid contents, liver histology and liver mRNA expressions of TNF-α, earnitine palmityl transferase - 1 （CPT-1）. acyl- COA earboxylase （ACC） and fatty acid synthase （FAS） were assayed at the end of the 12 th week. Results Compared with NAFLD control group, rosiglitazone-prevention and metformin-prevention groups serum TC（0. 55±0. 08,0. 71±0. 15 vs 0.85±0.15）mmol/L, ALT（49.1±7.4,56.0±33.6 vs 110. 6±44. 2）U/L, and liver TG（33.7±8. 2, 37.2±7.8 vs 54.8±7.6）mg/g decreased significantly （P〈0. 05） ; fatty denaturation of liver tissues and hepatic inflammation markedly reduced; and serum TNF-α（187.5±20. 3,211.9±26.6 vs 324.2±34. 2）pg/ml and liver TNF-α（0. 35±0. 12,0. 49±0. 18 vs 0. 85 ± 0. 20）, FAS mRNA （0. 34 ± 0. 13,0. 45 ± 0. 15 vs 0. 68± 0. 19）also decreased significantly （P〈0. 05）. Conclusions Rosiglitazone and metformin have protective effects on NAFLD in rats, and these effects may be related to the decreased expressions of TNF-α and FAS.
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
2007年第1期51-55,共5页
Chinese Journal of Geriatrics
基金
国家人事部博士后基金（2004036431）
关键词
脂肪肝
非酒精性
预防
胰岛素抵抗
Fatty liver, nonalcoholic disease
Prevention
Insulin resistance
