摘要
基于sm art技术构建了淫羊藿花蕾cDNA文库并检测了其质量。结果表明,该文库重组率为95%,平均插入片段大小为1095 bp,文库滴度为2×106pfu/mL,是一个高质量的淫羊藿花蕾cDNA文库。此文库的建立将有助于克隆与次生代谢相关的基因,特别是淫羊藿黄酮特异合成代谢的基因,其次是克隆与花发育相关的基因。
A eDNA library for flower buds of Epimedium brevicornu Maxim was constructed which based on SMART technology. Our results showed that the titer of unamplifed library from flower buds was 2×10^6pfu/mL. The percentage of recombinants was 95% and the average length of the insert eDNA fragments was 1095 bp. It suggested that one high quality eDNA library for flower buds of E. brevicornu were successfully constructed. Construction of eDNA library will help to clone the relevant genes associated with secondary metabolism, first, the special genes in the course of flavonoid biosynthesis; second, the genes related with development of flower buds in E. brevicornu Maxim.
出处
《武汉植物学研究》
CSCD
北大核心
2007年第1期105-108,共4页
Journal of Wuhan Botanical Research
基金
四川省杰出青年学科带头人培养计划项目(04ZQ026-047)资助
四川省科技厅应用基础项目(03JY029-021-22)资助
四川省重点学科建设项目(SZD0420)资助
关键词
CDNA文库
花蕾
淫羊藿
cDNA library
Flower buds
Epimedium brevicornu Maxim
