摘要
目的:研究白色念珠菌对口腔上皮细胞增殖及细胞周期的影响。从另一种角度证明白色念珠菌感染与癌发展的关系。方法:在培养的口腔上皮细胞(KB细胞)中,加入菌丝相、孢子相的白色念珠菌,共同培养48h,采用流式细胞仪观察白色念珠菌对KB细胞增殖及细胞周期的影响。结果:与对照组相比,菌丝组G0/G1期细胞所占比例降低,S期、G2/M期所占百分比显著升高,反映细胞增殖活力的增殖指数PI增高,差异有显著性(P<0.05)。而孢子组的KB细胞PI值与对照组相比无明显变化,P>0.05。结论:白色念珠菌可引起KB细胞细胞周期的改变;白色念珠菌对KB细胞周期的改变有赖于该菌的毒性及对细胞的感染。
Objective：To investigate the effects of Candida albicans （ C. albicans ） on the cell cycle distribution of oral epithelial KB cells in vitro. Methods：KB cells at 3 × 10^6/ml were seeded and cultured for 48 h, then the bacterial filament and the spore of C. albicans at 102 CFU/ml were added to the cultures respectively. KB cell culture without C. albicans was used as the control. After 48 h culture cell suspensions were prepared and subjected to FACS flow cy- tometry for detecting the cell cycle distribution. Results：Filament of C. albicans decreased the percentage of G0/G, cells, increased G2/M cell population and proliferation index of the cells（ P 〈 0.05 ）. Conclusions： C. albicans can change the cell cycle distribution of KB cells.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2007年第1期111-113,共3页
Journal of Practical Stomatology
