摘要
目的:探讨上皮性卵巢癌组织中环氧化酶-2(cyclooxygenase-2,COX-2)表达与卵巢癌化疗耐药和预后的关系,以及与p53表达的相关性。方法:采用免疫组化SP法检测54例上皮性卵巢癌组织和20例正常卵巢组织中COX-2和p53表达,分析其与上皮性卵巢癌临床病理因素、化疗耐药的关系,同时对预后进行多因素的Cox生存分析。结果:COX-2和p53在上皮性卵巢癌组织中阳性表达率分别为48.1%和59.3%,正常卵巢组织均未见表达,差异有极显著性(P<0.01);两者间表达呈显著正相关(P=0.01)。COX-2表达与患者病理类型、手术病理分期、病理分级及年龄无关,与术后残留灶直径有显著相关性(P<0.01)。COX-2、p53阳性表达率在耐药组和非耐药组之间相比有显著差异(P<0.01)。多因素生存分析显示,手术病理分期、COX-2表达和术后残留灶直径是影响患者预后的独立危险因素。结论:COX-2表达与上皮性卵巢癌组织化疗耐药相关,是影响患者预后的独立危险因素。COX-2表达与抑癌基因p53的突变可能具有相互促进作用,在卵巢癌化疗耐药中起重要作用。
Objective：To investigate the relationship between expression of cyclooxygenase-2 （COX-2） and chemotherapy resistance,progno- sis of patients with epithelial ovarian cancer, and to study the relationship between COX-2 and p53 expression. Methods：lmmunohistochemical assay （SP method） was used to detect the expressions of CL）X-2 and p53 in 54 epithelial ovarian cancer tissues and 20 normal ovarian tissues. Correlations between COX-2 and clinicopathological markers, chemotherapy resistance were analyzed. The prognosis of the patients with ovarian cancer was analyzed by Cox multivariate survival analysis. Results：The positive rates of COX-2 and p53 in 54 epithelial ovarian cancer tissues were 48. 1% and 59.3%, respectively. The expressions of COX-2 and p53 were not detected in normal ovarian tissues. The difference between epithelial ovarian cancer tissues and normal ovarian tissues was significant （P〈0. 01 ）. There was a significant positive correlation between COX-2 and p53 expression （P=0.01）. There was no correlation between CL）X-2 expression and pathohistological types, dinical stage, pathohistological grade and age. C-L）X-2 expression significantly correlated with the diameter of residual tumor （P〈0. 01）. There was significant difference in the positive rates of the expression of COX-2 and p53 between drug resistant group and drug sensitive group （P〈0. 01）. According to multivariate survival analysis, clinical stage, COX-2 expression, and the diameter of residual tumor were independent prognostic factors for poor prognosis （P=0.009）. Coneltuions：COX-2 expression appears to be closely related to the chemotherapy resistance of epithelial ovarian cancer. It is an independent prognostic factor for ovarian cancer patients. There may be an interaction between inactivation of antioncogene p53 and overexpression of COX-2, which plays an important role in the chemotherapy resistance of ovarian cancer.
出处
《肿瘤》
CAS
CSCD
北大核心
2007年第1期46-50,共5页
Tumor
基金
湖北省科技攻关项目(编号:2005AA304B05)
关键词
卵巢肿瘤
肿瘤
腺性和上皮性
抗药性
肿瘤
环氧化酶-2
蛋白质P53
预后
Ovarian neoplasms
Neoplasms, glandular and epithelial
Drug resistance, neoplasm
Cyclooxygenase-2
Protein p53
Prognosis
