摘要
目的:采用RNA干扰(RNA interference,RNAi)技术沉默胃癌细胞核因子-kappa B(nuclear factor-κB,NF-κB)亚单位p65后,观察其对细胞端粒酶活性的影响。方法:采用p65小干扰RNA(small interfering RNA,siRNA)转染胃癌细胞后,分别采用western blot和凝胶迟滞电泳检测胃癌细胞p65蛋白水平和NF-κB活性,采用MTT检测胃癌细胞增殖,采用TRAP-ELISA方法检测对胃癌细胞端粒酶活性。结果:转染组细胞p65蛋白和NF-κB活性明显被抑制,且成浓度依赖性。转染组胃癌细胞增殖明显受到抑制,呈浓度依赖性。TRAP-ELISA检测显示,转染组胃癌细胞端粒酶活性明显被抑制,且呈浓度依赖性。结论:针对NF-κB重要亚单位的p65 siRNA可抑制胃癌细胞端粒酶活性。
Objective： To study the effects of siRNA directed against the p65 subunit of nUclear factor- κB（NF-κB） on telomerase actisity of gastric cancer cell line. Methods： Gastric cancer AGS cells were transfected with different dose of p65 siRNA by use of oligofecamine. The expression of p65 protein and NF- κB activity were detected by western blot or electrophoretic mobility shift assay （EMSA）, respectively. The cell proliferation was determined by MTT assay. TRAP-ELISA assay was performed to evaluate the telomerase activity of cancer cells. Results： The expression of p65 protein and NF-KB activity of tranfected cell were down-regulated significantly in a dose-dependent manner. The growth of gastric cancer cell inhibited in a dose-dependent manner. The telomerase activity of transfected cancer cells inhibited in a dose-and timedependent manner. Conclusion： These data indicated that siRNA directed against the p65 subunit of NF- B might suppress telomerase activity of gastric cancer cell.
出处
《江苏大学学报（医学版）》
CAS
2007年第1期43-45,共3页
Journal of Jiangsu University：Medicine Edition
基金
镇江市科技计划项目(SH2006019)
