摘要
本实验用抗绿脓杆菌ｉ－ＲＮＡ致敏小鼠，用酶联免疫吸附试验直接检测鼠体内绿脓杆菌特异性抗体的产生，并通过电镜技术观察补体参与下的溶菌杀菌现象来间接反映体内是否有特异性抗体的产生。结果证实ｉ－ＲＮＡ没有传递特异性体液兔疫活性的能力。但小鼠体内脾细胞抗体产生能力，巨噬细胞吞噬活性，脾细胞ＮＫ活性和ＩＬ－２活性均明显高于正常鼠，提示抗菌ｉ－ＲＮＡ有佐剂活性，可以增强机体的体液免疫功能和细胞免疫功能。
Serum antibody were determined by ELISA. immunoelectromicroscopytechnique in mice immunized with Pa-iRNA. It was shown that corresponding serum antibodies were not found in Pa-iRNA-immunized group.However. it was. demonstrated that antibody productivity, engulfmentactivity of macroghages, NK activity of spleen cells and IL-2 activityin iRNA-immunized groups were all significantly higher than that innormal group. So it can be proyed that Pa-iRNA has adjuvant activity,and it can enhance humoral immunity of the organism and immunity ofthe cells.
出处
《微生物学杂志》
CAS
CSCD
1996年第4期18-22,共5页
Journal of Microbiology
