摘要
目的应用脊髓片器官型培养模型制备选择性运动神经元死亡的肌萎缩侧索硬化(ALS)体外模型,探讨星形胶质细胞在ALS发病中的作用。方法脊髓器官型培养采用8日龄SD大鼠的腰段脊髓,在培养液中加入浓度为100μmol/L的苏-羟天冬氨酸(THA),建立ALS脊髓器官型培养模型;用神经元特异的SMI-32和星形胶质细胞特异的GFAP染色,并对细胞计数。结果与对照组相比,THA100μmol/L组的脊髓前角以及周边的白质中星形胶质细胞明显增生,增生的程度随用药时间的延长而增加,且伴随细胞数量的增加,其形态也发生改变。结论THA干预制造的ALS模型中,在前角位置有明显的星形胶质细胞增生,且增生的时间明显早于运动神经元的丢失。
Objective To establish the model of amyotrophic lateral sclerosis （ALS） with selective motor neuron disorder by organotypic spinal cord cultures, and analyze the role of astrocyte in the pathagenisis of ALS. Methods Organotypic spinal cord cultures were prepared using lumbar spinal cord slices from 8-day-old SD rat pups. The threohydroxyaspartate （THA） was applied into culture medium to establish ALS organotypic spinal cord cultures model. Motor neurons survival was evaluated by monoclonal SMI-32 immunohistochemical staining and glial fibrillary acidic protein staining to show astrocyte survival. Results Compared with the control group, there was significantly astrogliosis in the anterior horn and surrounding white matter in THA 100μmol/L group, and the level of gliosis was increased followed the elongation of THA interference time. With the increasing of the number of astrocyte, the morphology of astrocyte was changed. Conclusion There is significantly astrogliosis in the anterior horn and the time of astrogliosis is markedly earlier than the time of motor neuron loss in the AIrS model intervented with THA.
出处
《中国康复理论与实践》
CSCD
2006年第12期1052-1053,F0003,共3页
Chinese Journal of Rehabilitation Theory and Practice
关键词
肌萎缩侧索硬化
器官型培养
星形胶质细胞
amyotrophic lateral sclerosis （ALS）
organizational cultures
astrocytes
