摘要
对无环鸟苷和鸟嘌呤的高效毛细管电泳（HPCE）分离测定条件进行了详细研究，建立了以2－氨基－5－巯基－3，4－噻二唑为内标的快速、灵敏的HPCE定量分析方法。当无环鸟苷和鸟嘌呤的浓度分别在10～300mg／L和5～300mg／L范围时，可进行定量分析，最低检出限分别为8．52mg／L和2．80mg／L。该方法用于无环鸟苷产品的质量检测，结果准确。用于尿液中的无环鸟苷和鸟嘌呤的快速测定，相对标准偏差小于4．5％。无环鸟苷和鸟嘌呤的回收率分别优于81％和86％。
In this paper, the separation and determination conditions of acyclic guanosine and guanine by high performance capillary electrophoresis have been studied systematically. A simple and reproducible HPCE method for determining acyclic guanosine and guanine in acyclic guanosine product and urine is presented. 2-amino-5-mercapto-3, 4-dithiazole was used as an internal standard. Results showed that the linear ranges of determination for acyclic guanosine and guanine are 10~300 mg/L and 5-300 mg/L, and the detection limits are 8. 52mg/L and 2. 80 mg/L (S/N= 2) respectively. The recoveries are more than 81% for acyclic guanosine and 86% for guanine in urine. RSD is less than 4. 5%.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1996年第10期1212-1215,共4页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金
中国科学院长春应用化学研究所电分析化学开放研究实验室基金
关键词
高效毛细管电泳
无环鸟苷
鸟嘌呤
尿
High performance capillary electrophoresis, acyclic guanosine, guanine, urine
