摘要
通过驯化富集培养,从红树林底泥中分离出6株硝基苯酚降解菌,其中Rhodococcussp.Ns为对硝基苯酚(PNP)与邻硝基苯酚(ONP)的高效降解菌.在好氧条件下该菌可以耐受小于1.8 mmol/L的PNP,能够利用PNP和ONP为唯一碳源、能源和氮源生长并将其完全矿化.研究了Rhodococcussp.Ns在不同pH、盐度与浓度范围下,PNP的降解特性并探讨了该菌降解PNP的途径.实验得出该菌在盐度<5‰、pH>5的条件下能较快生长,1.5 mmol/L的PNP在96h内被完全降解,并检测到至少2种中间产物4-硝基儿茶酚(4-nitrocatechol)和1,2,4-苯三酚(1,2,4-benzenetriol).红树林底泥中固有的细菌对PNP和ONP具有高效降解作用.
A bacterium capable of utilizing p-nitrophenol （PNP） as the sole nitrogen, carbon, and energy source was isolated from mangrove sediment. This bacterium was confirmed based on 16S rDNA sequence analysis and taxonomic analysis as being a member of the genus Rhodococcus and was designated strain Ns. The strain Ns could tolerate high concentrations of PNP up to 1.5 mmol/L and complete degradation was achieved in four days at 30℃ in the dark under aerobic conditions. Biodegradation of PNP quickly occurred at optimal pH above 5.0, and at least 5‰ salinity. Bacterial growth on PNP was observed with the simultaneous production of 4-nitrocatechol, which was also degraded by the same bacterium, as a degradation intermediate, Moreover, Rhodococcus sp. Ns was able to also deplete 2-nitrophenol（ONP） as the sole source of carbon, nitrogen and energy, but not 3-nitrophenol. The experimental results show that bacteria indigenous to the natural wetland sediment are capable of complete degradation of PNP and ONP.
出处
《环境科学》
EI
CAS
CSCD
北大核心
2007年第2期431-435,共5页
Environmental Science
基金
国家高技术研究发展计划(863)项目(2002AA601160)
国家自然科学基金委员会-水利部黄河水利委员会联合研究基金项目(50239010)
