摘要
研究1株碱性脂肪酶产生菌L198发酵条件及酶学性质。结果表明，产酶发酵培养基为（％）：豆饼粉1．0。玉米浆1．0，葡萄糖1．0，K2HPO40．5，NaNO30．5。500mL三角瓶装液50mL，30℃，180r／min摇床培养40h。酶学性质研究：最适反应温度为40℃。最适pH9．0；在pH8～10稳定。其热稳定性较好，在20～40℃放置6h后仍可以保持较好酶活力。金属离子Na^＋、K^＋对酶活有显著激活作用，而Cu^2＋对酶活有显著抑制作用，而K^＋、Mg^2＋、Fe^＋对酶活有抑制作用。
An alkaline lipase - producing strain L198 was studied. The optimal medium composition and enzymatic properties were determined. The optimal medium composition for the lipase production is （ %） ： soybean flour 1.0, corn milk 1.0, glucose 1.0, K2HPO4 0.5, NaNO3 0.5. Culture medium volumes for 50 mL flask were cultivated aerobically at 20℃ with an aeration rate of 180 r/min for 40 hours. The enzymatic properties were concluded. The optimum temperature and pH for it was 40℃ and 9.0. It was stable in the pH range from 8 -10 and had good thermal stabilities- above 80% enzyme activity remained after incubated in the temperature range from 20-40℃ for 6 hours. The lipase activity was stimulated in the presence of metal ions such as Na^＋, K^＋ ,but was inhibited by Cu^2＋ ,K^＋ ,Mg^2＋ ,Fe^＋.
出处
《新疆农业科学》
CAS
CSCD
2007年第1期87-90,共4页
Xinjiang Agricultural Sciences
基金
国家"863"项目(2004AA227110)
关键词
碱性脂肪酶
产酶条件
酶学性质
alkaline lipase
fermentation condition
enzymatic property
