摘要
目的:研究白血病细胞纤溶活性及其与尿激酶受体(uPAR)和膜联蛋白Ⅱ(annexinⅡ)表达的关系。方法:用发色底物法测定NB4、SHI-1、K562、Jurkat、Raji细胞与纤溶酶原相互作用后白血病细胞的纤溶活性。用流式细胞术检测NB4、SHI-1、K562、Jurkat、Raji细胞表面uPAR和annexinⅡ的表达。以RT-PCR方法检测uPAR基因和annexinⅡ基因在NB4、SHI-1、K562、Jurkat、Raji细胞系中的表达。结果:SHI-1细胞和NB4细胞的纤溶活性明显高于Raji、K562和Jurkat细胞的纤溶活性。uPAR和annexinⅡ在NB4细胞的表达率分别为(13.15±1.61)%与(95.97±1.19)%,在SHI-1细胞的表达分别为(99.00±0.26)%与(90.35±2.15)%,在K562、Jurkat、Raji细胞的表达均低。NB4细胞annexinⅡ的基因表达较SHI-1细胞高,K562和Jurkat细胞中未检测到annexinⅡ的基因表达。NB4和SHI-1细胞uPAR基因表达高于Jurkat和K562细胞,Raji细胞中未检测到uPAR基因的表达。结论:NB4与SHI-1白血病细胞株存在高纤溶活性,与annexinⅡ和uPAR的表达增高相关,这可能是早幼粒细胞白血病与急性单核细胞白血病患者在临床上易发生出血及并发弥散性血管内凝血的一个重要原因。
AIM： To study the relation between expression of uPAR and annexin Ⅱ and fibrinolytic activity in various leukemic cell lines. METHODS： The plasma activity was measured under the reaction between cells of NB4, SHI - 1, K562, Jurkat, Raji and plaminogen by chromogenic assay. The protein expressions of uPAR and annexin Ⅱ in cells of NB4, SHI - 1, K562, Jurkat, Raji were detected by flow cytometry method. The mRNA expressions of uPAR and annexin Ⅱ in cells of NB4, SHI -1, K562, Jurkat, Raji were detected by RT - PCR. RESULTS ： The plasma activity in SHI - 1 cells and NB4 cells were higher obviously than that in Raji, K562 and Jurkat cells. The protein expression ratio of uPAR and annexin Ⅱ in NB4 cells were （ 13. 15 ± 1.61 ）% and （95.97 ± 1.19）% , respectively, they were （99. 00 ± 0. 26）% , （90. 35 ±2. 15）% respectively in SHI - 1 cells, and they were lower in K562, Jurkat, Raji cells. The expression of annexin Ⅱ mRNA in NB4 cells was higher than that in SHI - 1 cells, and they were undectectable in K562 and Jurkat cells. The expression of uPAR mRNA in NB4 and SHI - 1 cells were higher than that in Jurkat and K562 cells. The expression of uPAR mRNA in Raji cells was undectectable. CONCLUSION： The primary hyperfibrinolysis in leucocylhemia cells was observed, and relation was closely with the expression of annexinⅡ. It might be the main reason for bleeding and disseminated intravasculax coagulation in patients with acute promyelocytic leukemia and acute monocytic leukemia.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2007年第2期250-253,共4页
Chinese Journal of Pathophysiology
基金
江苏省卫生厅科技基金会重点科研课题资助项目(No.k200401)
关键词
白血病
早幼粒细胞
急性
受体
尿激酶
基因表达
Leukemia, promyelocytic, acute
Receptors, urokinase
Gene expression
