摘要
目的检测喉鳞癌细胞 Hep-2对 TRAIL 诱导的凋亡敏感性及细胞表面 TRAIL4个受体的表达差异。方法 CCK-8法检测 TRAIL 对 Hep-2细胞的生长抑制率,流式细胞仪检测 Hep-2细胞表面 TRAIL 受体表达及细胞凋亡率。结果 Hep-2细胞对 TRAIL 诱导的细胞凋亡敏感性低,24h IC_(50)为596.92ng/mL。Hep-2细胞表面诱骗受体(DcR1 80.81%、DcR2 62.65%)表达明显高于死亡受体(DR4 60.26%、DR5 30.06%)。结论单用TRAIL 不适用于喉鳞癌的治疗,应寻求合理的联合用药策略。(中国眼耳鼻喉科杂志,2007,7:20～21)
Purpose To investigate the sensitivity of Hep-2 cells of the laryngeal squamous carcinoma to apoptosis induced by TRAIL and the expressions of TRAIL receptors in Hep-2 cells. Methods The inhibition rate of Hep-2 cells induced by TRAIL was measured by CCK-8 assay. The apoptosis rate of Hep-2 cells and the expressions of DR4, DRS, DcRI and DcR2 in Hep-2 cells were detected by flow cytometry methods. Results The Hep-2 cell was resistant to apoptosis induced by TRAIL and the IC50（24 h） was 596.92 ng/mL. The expressions of decoy receptors were significantly higher than death receptors in Hep-2 cells. Conclusion Hep-2 cells are resistant to TRAIL because of high expressions of decoy rerceptors. TRAIL is not effective enough to cure the laryngeal squamous carcinoma. Finding new strategy of combinative administration of TRAIL and chemotherapeutic drugs has become the next research direction. （Chin J Ophthalmol and Otorhinolaryngol,2007,.7 ： 20-21）
出处
《中国眼耳鼻喉科杂志》
2007年第1期20-21,30,I0002,共4页
Chinese Journal of Ophthalmology and Otorhinolaryngology
基金
上海市优秀学科带头人计划(06XD14031)
关键词
喉肿瘤
肿瘤坏死因子相关诱导凋亡配体
凋亡
Laryngeal neoplasma
Tumor necrosis factor-related apoptosis-inducing ligand
Apoptosis
