摘要
目的:观察关木通引起大鼠肾损伤时血和尿中尾加压素Ⅱ(UⅡ)含量的变化。方法:雄性Wistar大鼠随机分为对照组(5只)和给药组(25只)。大鼠灌胃给予关木通水煎剂后0、3、7、15、25 d和停药10 d时采血并留取24 h尿液,分别检测血肌酐、尿素氮、24 h尿蛋白、尿视黄醇结合蛋白(RBP)、尿N-乙酰-β-氨基葡萄糖苷酶(NAG)水平及血浆、尿UⅡ含量;观察上述时间点肾脏组织病理学改变。结果:①给予关木通后不同时间血肌酐、尿素氮及24 h尿蛋白含量与对照组比较无明显升高;与对照组比较,尿NAG于给药15 d后明显升高(P<0.05),给药25 d后NAG及RBP含量均增加(P<0.01),停药10 d两者含量与25 d时比较未见明显下降。②与对照组比较,血UⅡ含量在给予关木通15 d即明显升高(P<0.05),25 d达峰值(P<0.01),停药10 d仍持续在较高水平(P<0.01);尿UⅡ含量于给药25 d时较对照组明显升高(P<0.05),停药10 d时仍在较高水平(P<0.05)。③给药3 d后HE染色可见局部肾小管上皮细胞变性、坏死和崩解,并随给药时间的延长逐渐加重,停药10 d病变较25 d时有所减轻。结论:关木通所致肾损伤可引起血、尿UⅡ含量明显升高,提示UⅡ在关木通所致肾损伤的发生发展中可能有一定的作用。
Objective To observe the alterations of urotensinⅡ （U Ⅱ ） contents in blood and urine in Caulis Aristolochiae Manshuriensis （CAM） -induced renal lesions in rats. Methods Male Wistar rats were divided into two groups ： control group （n= 5） and administration group （n=25）. After CAM were administered by gavage to rats for 0, 3, 7, 15, 25 d, and 10 d after withdrawal of CAM, the blood and 24 hour-urine samples were collected, 24 hour-urinary protein excretion, urinary retinol-binding protein （RBP）, N-acetyl-D glucosaminidase NAG）, serum creatinine （Scr） and blood urea nitrgen （BUN） were examined, and the plasma and urinary U Ⅱcontents were detected. The pathological changes in kidney were observed at these various time. Results （1） At various time after administration of CAM, the Scr , BUN and 24 hour-urinary protein excretion levels were not increased than those in control group. Compared with control group, after administration of CAM for 15 d, urinary NAG content was significantly higher （P〈0.05）. After administration of CAM for 25 d, urinary NAG and RBP contents were significantly higher （P〈0.01）. After CAM was withdrawn for 10 d, the urinary RBP and NAG contents were not decreased compared with those at 25th day after administration. （2） Compared with control group, after administration of CAM for 15 d, the plasma UⅡ content was significantly increased （P〈0.05）; and after administration of CAM for 25 d, the plasma UⅡ content was peak value （P〈0.01）; 10 d after withdrawal of CAM, plasma UⅡ content was higher （P〈0.01）. After CAM-treated for 25 d, the urinary UⅡ content was increased compared with controls （P〈0.05） ; and 10 d after withdrawal of CAM, the urinary UⅡ content was increased still （P〈20.05）. （3） The degeneration and necrosis in tubules was the major pathological changes in rats after administration of CAM for 3 d, and the pathological changes were aggravated as treatment time increased. After CAM was withdrawn for 10 d, the pathological changes were alleviated than 25 d. Gonclusion The plasma and urinary UⅡ contents are significantly elevated in CAM induced renal lesions in rats, suggesting that UⅡ may play a pathological role in the development of renal lesions.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2007年第1期21-24,F0002,共5页
Journal of Jilin University：Medicine Edition
基金
国家自然科学基金资助课题(30570855)
关键词
尾加压素Ⅱ
肾/损伤
关木通
urotensin Ⅱ
kidney / injuries
Caulis Aristolochiae Manshuriensis
