摘要
小鼠颈背部皮下注射D-半乳糖生理盐水溶液(125 mg/(kg.d))诱导其衰老,同时分别灌胃10,20和40 mg/(kg.d)玉米幼芽提取物(Extract of maize plumule,EMP),每日1次,连续处理42 d后,测定心肌组织超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活性及丙二醛(MDA)和一氧化氮(NO)的含量,并用透射电镜观察心肌细胞超微结构,研究EMP对D-半乳糖致衰老小鼠心肌自由基代谢和超微结构的影响。结果表明,与对照组相比,D-半乳糖能明显的降低小鼠心肌组织中的SOD、CAT和GSH-Px活性,提高MDA、NO含量;与衰老模型组比较,20 mg/kg EMP处理组小鼠心肌组织的SOD、CAT和GSH-Px活性均极显著升高,MDA和NO含量均极显著降低;与对照组相比,衰老模型组小鼠的心肌Z线变宽、模糊,肌原纤维断裂、溶解、凝集,线粒体肿胀、空泡化、嵴消失;20 mg/kg EMP处理组的以上病变均有明显的改善。这表明20 mg/kg的EMP具有增强衰老小鼠心肌抗氧化酶活性,改善心肌自由基代谢,保护心肌细胞超微结构的作用。
Effect of extract of maize plumule （EMP） on free radical metabolism and ultrastructure of myocardium in aging mice induced by D-galactose was investigated. Physiological saline solution of D-galactose was applied in subcutaneous injection on the back of neck 125 mg/kg per day,once daily continuously for 42 days to induce the aging model. At the same time,EMP （10,20 and 40 mg/kg） was given by gastric perfusion once daily continuously for 42 days too to study the activity of superoxide dismutase （SOD）, Catalase （CAT）, Glutathione peroxidase （GSH-Px） and the content of malondialdehyde （MDA） and nitric oxide （NO）. Meanwhile,the ultrastructure of myocardium was observed under transmission electron microscope. The data show that D-galactose caused a significant decrease in the SOD,CAT and GSH-Px activity and increase in MDA and NO content versus control group. Compared with model group,mice treated with both D-galactose and EMP （20 mg/kg） obviously had a higher SOD,CAT and GSH-Px activity and lower MDA and NO content. Under transmission electron microscope,these pathological changes were observed in aging model group ：Z-line broadening and illegibility; Myofilament fragmentation,dissolution, agglutination; Mitochondrion swelling, vacuolization, cristae vanishing. These pathological changes were significantly improved by EMP at a concentration of 20 mg/kg. The result indicates that 20 mg/kg EMP can increase the activity of anti-oxidation enzyme,improve free radical metabolism and protect ultrastructure of myocardium from oxidative stress in aging mice.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2007年第2期23-26,共4页
Journal of Northwest A&F University(Natural Science Edition)
基金
西北农林科技大学校长专项基金项目(130709)
关键词
玉米幼芽提取物
D-半乳糖
抗氧化酶
自由基代谢
extract of maize plumule （EMP）
metabolism D-galactose
anti-oxidation enzyme
free radical
