摘要
在4℃避光条件下，将藻蓝蛋白（简称PC）与AgNO3作用，通过UV，FS，FTIR等谱学方法研究了PC与Ag（Ⅰ）原位还原与纳米Ag（0）粒子形成的动态过程。结果显示：PC在615nm的特征吸收峰强度明显减弱，并随Ag（Ⅰ）浓度增加和时间延长单调降低；PC的荧光发射峰和荧光激发峰也均呈现衰减趋势。同步荧光光谱观察到纳米Ag（0）粒子形成的动态过程。用TEM观察到所形成的Ag（0）分散于PC表面，形成PC为核，纳米银为壳部分包覆的生物缀合物，粒子呈球形，有较窄的分布尺寸，粒径在15～30nm之间。
The interaction of Ag（ Ⅰ） and Phycocyanin（PC for short） was investigated by ultraviolet-visible, fluorescence and infrared spectra under the condition of 4℃ and without light. The results showed that the characteristic absorption of PC at 615 nm decreased gradually with the increase in Ag（ Ⅰ ） concentration and interaction time. Accordingly, its fluorescence emission peak and two fluorescence excitation peaks also decreased. Dynamic formation of nano-Ag（0） was also observed by synchronous fluorescence spectra, showing that Ag（0） was formed by the PC in situ reduction. It was observed by TEM that Ag（0） dispersed in PC, which formed a bioconjugate with PC as the core and nano-Ag（0） as the crust. The bioeonjugate was round and its diameter was about 15-30nm.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2007年第1期23-27,共5页
Spectroscopy and Spectral Analysis
基金
广东省自然科学基金重点项目(05103295)
广东省自然科学基金团队项目(039213)资助
关键词
银
钝顶螺旋藻
藻蓝蛋白
光谱
纳米
Silver
Spirulina platensis
Phycocyanin
Spectroscopy
Nanoparticle
