摘要
利用面阵CCD摄像机采集显微镜中孢子的图像,通过灰度直方图确定初始阈值,再用二次定值法确定最终阈值,将灰度图像转换成二值图像。扫描图像,计算并比较孢子区域各像素点的图像距离,确定各个孢子的中心,实现从图像中对孢子自动计数。
The images of spores under microscope were captured by area-scan CCD camera, and the gray level images were then transferred into binary images by adjusting threshold. The distances of all pixels in the spore＇s zone were scanned and calculated by comparing their distances. The center of every spore was identified. Thus the number of these spores could be counted.
出处
《植物保护》
CAS
CSCD
北大核心
2007年第1期109-112,共4页
Plant Protection
