摘要
为了探讨各弓形虫株间毒力、致病性以及在分子水平上的差异，对ＲＨ株、ＰＰ株、ｓｈ－１株弓形虫毒力进行了观察，结果ＲＨ株对鼠毒力致病性最强，ｓｈ－１株次之，ＰＰ株最弱。三株弓形虫基因组ＤＮＡ经ＨｉｎｄＩＩ、ＢａｍＨＩ、ＳａｌＩ酶切后电泳，结果呈涂布状，用ＥｃｏＲＩ酶切后，三株弓形虫ＤＮＡ均出现一条带，说明弓形虫株间分化程度不高，采用酶切分析法不能分辨出株间差异，应在此基础上用单考贝或多考贝的探针进行Ｓｏｕｔｈｅｒｎ印迹分析，或用更先进的分辨力更高的方法进行分析。
In this paper,we observed pathogenicity of three strains T.gondii.The results was demonstrated that RH strain T.gondii is virulent,Sh 1 strain is intermediate,PP strain is chronic.Three strains T.gondii genomic DNA was cleaved with endonuclease HindIII.BamHI.SalI.It was shown smear by using electrophoretic analysis.Three strains T.gondii genomic DNA cleaved with endonuclease EcoRI was shown only a band on agarose gel electrophoresis,suggesting degree of differentiation among toxoplasmic strains is low.The difference strain was indistinguishable by RFLP analysis.We consider that Southern blot or more advanced method should be adopted.
出处
《天津医科大学学报》
1996年第4期10-11,18,共3页
Journal of Tianjin Medical University
基金
天津医科大学青年基金
关键词
弓形虫
株间差异
寄生虫学
Toxoplasma gondii
The difference strain
RFLP
