摘要
The diameter of the excitation beam was decreased greatly by integrating the fiber on the microfluidic chip as light propagation medium.The coupling efficiency of the fiber was improved with optical fiber collimation device coupling beam. The chip was placed in the darkroom to avoid the interference of the external light.The cost of the instrument was decreased with a high brightness blue LED as excitation source;the performance of the system was valuated by the determination of FITC fluorescein with a minimum detectable concentration of 2.2×10^(-8) mol/L,the Signal-to-Noise Ratio (SNR) S/N=5.The correlation coefficient of the detection system within the range of 1.8×10^(-7) mol/L～4×10^(-5)mol/L was 0.9972.
The diameter of the excitation beam was decreased greatly by integrating the fiber on the microfluidic chip as light propagation medium. The coupling efficiency of the fiber was improved with optical fiber collimation device coupling beam. The chip was placed in the darkroom to avoid the interference of the external light. The cost of the instrument was decreased with a high brightness blue LED as excitation source; the performance of the system was valuated by the determination of FITC fluorescein with a minimum detectable concentration of 2.2×10^-8 mol/L, the Signal-to-Noise Ratio （SNR） S/N=5. The correlation coefficient of the detection system within the range of 1.8× 10^-7 mol/L- 4×10^-5mol/L was 0.9972.
出处
《稀有金属材料与工程》
SCIE
EI
CAS
CSCD
北大核心
2006年第A03期525-526,共2页
Rare Metal Materials and Engineering
基金
financial support from the National Science Foundation of China under Grant number 20299030,60427001 and 60501020.
