摘要
人工合成Ｍ１和Ｍ２胆碱受体蛋白的细胞外肽段，联接血蓝蛋白后免疫家兔，制备出Ｍ１和Ｍ２受体的亚型特异性抗体．用此抗体观察脑室内注射Ａｌ－Ｃｌ３４０μｇ引起的学习记忆障碍小鼠部分脑区Ｍ受体的含量变化，发现实验组小鼠海马Ｍ１和Ｍ２受体数降低而顶叶皮层。
The subtype specific antibodies against the synthetic peptides corresponding to sequence of the extracellular polypeptides of M 1 and M 2 subtypes of muscarinic acetylcholinergic receptors (mAChR) were prepared. The reactivity and specificity for different synthesized peptides and rat brain tissues of two different antibodies were tested by enzyme linked immunosorbent assay (ELISA). The results suggest that the antibodies are characterized by high specificity and high reactivity to mAChR subtypes. In the mice whose learning and memory ability were inhibited by AlCl 3(40 μg, icv), the changes of M 1 and M 2 receptors contents in some brain regions were measured with ELISA by these antibodies. It was found that the contents of M 1 and M 2 receptor in hippocampus were decreased significantly but those in parietal cortex, frontal cortex and brain stem remained unchanged.
出处
《中国药理学与毒理学杂志》
CSCD
北大核心
1996年第3期215-218,共4页
Chinese Journal of Pharmacology and Toxicology
基金
国家自然科学基金
关键词
受体
毒蕈碱
M肥碱
氯化铝中毒
receptors
muscarinic
antibodies
enzyme linked immunosorbent assay
brain
aluminum
memory disorders
