摘要
目的评价日本血吸虫DNA疫苗pcDNA3/SjSDISP的安全性。方法同体左右侧自身对比法对3只家兔进行局部肌肉刺激实验,22只豚鼠随机分为4组:A组为无菌生理盐水阴性对照组,B组为牛血清白蛋白阳性对照组,C组为pcDNA3/SjSDISP低剂量组,D组为pcDNA3/SjSDISP高剂量组,进行全身过敏实验。结果日本血吸虫DNA疫苗pcDNA3/SjSDISP对家兔局部肌肉刺激反应轻微,对豚鼠无全身过敏反应。结论日本血吸虫DNA疫苗pcDNA3/SjSDISP在该实验条件下安全。
Objective To evaluate the security of DNA vaccine pcDNA3/SjSDISP. Methods Three rabbits were tested for local muscle stimulation with intra-individual left/right self comparison method, and 22 guinea pigs were divided into 4 groups at random（A, physiological saline group; B, bovine serum albumin group; C, low dosage of pcDNA3/SjSDISP; D, high dosage of pcDNA3/SjSDISP） and were tested for systemic anaphylaxis of the vaccine. Results The DNA vaccine pcDNA3/SjSDISP had mild muscle stimulation and no allery effect. Coneluslon The DNA vaccine pcDNA3/SjSDISP is safe under the experimental condition.
出处
《中国血吸虫病防治杂志》
CAS
CSCD
2007年第1期21-23,共3页
Chinese Journal of Schistosomiasis Control
基金
"十五"国家科技攻关重大专项(2002AA2Z3343)
国家高技术研究发展计划(863计划)(2004AA2Z3530)
湖南省"十一五"重大专项(2006SK1001)
湖南省重点学科建设专项(03-985-3)
