摘要
目的：建立黄芪精口服液中芒柄花素的含量测定方法。方法：采用高效液相色谱法，色谱柱：十八烷基硅烷键合硅胶为填充剂（romasil 5-C^18，250mm×4．6mm，5μm）；流动相：甲醇-水（60：40）；流速：1ml·min^-1；检测波长：249nm。结果：芒柄花素在0．02—0．36嵋范围内，其进样量与峰面积呈良好线性关系。芒柄花素的平均回收率为99．14％，RSD=0．87％。结论：该方法准确，重复性好，可对该制剂进行含量测定及质量控制。
Objective： To establish the method for determination of the fermononetin in Huangqijing oral liquid. Method： The HPLC was adopted, the chromatographic conditions included romasil 5 -C,scolumn（250 mm×4.6 mm,5 μm） and mobile phase consisted of methanol - water （ 60 ： 40 ） at the flow rate of 1 ml/min, the detection wavelength was 249 nm. Result ： the fermononetin showed a good linear relationship at the range of 0.02 - 0. 36μg. The average recovery of fermononetin was 99. 14% with RSD 0.87%. Conclusion： The method is accurate with good reproducibility, can be used to determine content of the preparation and control its quality.
出处
《中医药导报》
2007年第1期66-67,共2页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
